Single-cell RNA sequencing of psoriatic skin identifies pathogenic Tc17 cell subsets and reveals distinctions between CD8
Autoimmunity
CD8-Positive T-Lymphocytes
/ immunology
Case-Control Studies
Gene Expression Profiling
High-Throughput Nucleotide Sequencing
Humans
Immunologic Memory
Immunophenotyping
Interleukin-17
/ biosynthesis
Neoplasms
/ genetics
Psoriasis
/ etiology
Single-Cell Analysis
T-Lymphocyte Subsets
/ immunology
CD8(+) T cell
Single-cell RNA sequencing
Smart-seq2
T-cell exhaustion
melanoma
psoriasis
Journal
The Journal of allergy and clinical immunology
ISSN: 1097-6825
Titre abrégé: J Allergy Clin Immunol
Pays: United States
ID NLM: 1275002
Informations de publication
Date de publication:
06 2021
06 2021
Historique:
received:
01
04
2020
revised:
23
10
2020
accepted:
19
11
2020
pubmed:
15
12
2020
medline:
5
10
2021
entrez:
14
12
2020
Statut:
ppublish
Résumé
Psoriasis is an inflammatory, IL-17-driven skin disease in which autoantigen-induced CD8 Our study focused on comprehensively characterizing the phenotypic variation of CD8 We used single-cell RNA sequencing to compare CD8 We identified 11 transcriptionally diverse CD8 Using high-resolution single-cell profiling in tissue, we have uncovered the diverse landscape of CD8
Sections du résumé
BACKGROUND
Psoriasis is an inflammatory, IL-17-driven skin disease in which autoantigen-induced CD8
OBJECTIVE
Our study focused on comprehensively characterizing the phenotypic variation of CD8
METHODS
We used single-cell RNA sequencing to compare CD8
RESULTS
We identified 11 transcriptionally diverse CD8
CONCLUSION
Using high-resolution single-cell profiling in tissue, we have uncovered the diverse landscape of CD8
Identifiants
pubmed: 33309739
pii: S0091-6749(20)31702-4
doi: 10.1016/j.jaci.2020.11.028
pmc: PMC9179181
mid: NIHMS1802448
pii:
doi:
Substances chimiques
Interleukin-17
0
Types de publication
Journal Article
Research Support, N.I.H., Extramural
Research Support, Non-U.S. Gov't
Langues
eng
Sous-ensembles de citation
IM
Pagination
2370-2380Subventions
Organisme : NIAMS NIH HHS
ID : R01 AR075864
Pays : United States
Organisme : NIAMS NIH HHS
ID : T32 AR007175
Pays : United States
Organisme : NIAID NIH HHS
ID : U01 AI119125
Pays : United States
Informations de copyright
Copyright © 2020 American Academy of Allergy, Asthma & Immunology. Published by Elsevier Inc. All rights reserved.
Références
Sci Transl Med. 2015 Mar 18;7(279):279ra39
pubmed: 25787765
Front Immunol. 2018 Jul 06;9:1549
pubmed: 30034395
Am J Pathol. 1997 Nov;151(5):1321-9
pubmed: 9358758
Cancer Immunol Res. 2019 May;7(5):784-796
pubmed: 30872264
J Invest Dermatol. 2010 Jul;130(7):1829-40
pubmed: 20220767
Nat Methods. 2018 Apr;15(4):255-261
pubmed: 29481549
Lancet. 2017 Jul 15;390(10091):276-288
pubmed: 28596043
Nat Biotechnol. 2014 Sep;32(9):896-902
pubmed: 25150836
J Immunol. 2009 Dec 1;183(11):7161-8
pubmed: 19917680
Bioinformatics. 2015 Jan 15;31(2):166-9
pubmed: 25260700
N Engl J Med. 2012 Mar 29;366(13):1181-9
pubmed: 22455412
Cell. 2015 Sep 10;162(6):1229-41
pubmed: 26321679
J Leukoc Biol. 2009 Aug;86(2):435-43
pubmed: 19487306
J Clin Invest. 2016 Oct 3;126(10):3905-3916
pubmed: 27617863
Annu Rev Immunol. 2019 Apr 26;37:457-495
pubmed: 30676822
Nat Commun. 2015 Mar 26;6:6692
pubmed: 25809635
Front Immunol. 2019 Jun 18;10:1322
pubmed: 31275308
Immunity. 2017 Feb 21;46(2):287-300
pubmed: 28214226
Nat Methods. 2016 Apr;13(4):329-332
pubmed: 26950746
Arch Dermatol Res. 1994;286(2):87-96
pubmed: 7512323
Cell. 2019 Feb 7;176(4):775-789.e18
pubmed: 30595452
Immunity. 2016 Aug 16;45(2):358-73
pubmed: 27496729
Oncoimmunology. 2017 Jan 20;6(2):e1273300
pubmed: 28344884
Ann Rheum Dis. 2007 Jun;66(6):720-6
pubmed: 17223654
J Invest Dermatol. 2008 May;128(5):1207-11
pubmed: 18200064
Nat Commun. 2014 Dec 03;5:5621
pubmed: 25470744
J Am Acad Dermatol. 2017 Mar;76(3):405-417
pubmed: 28057360
J Invest Dermatol. 2014 May;134(5):1462-1465
pubmed: 24401998
J Clin Invest. 2017 Nov 1;127(11):4031-4041
pubmed: 28945199
PLoS Biol. 2009 Mar 10;7(3):e54
pubmed: 19278296
J Immunol. 2006 Apr 1;176(7):4431-9
pubmed: 16547281
Br J Dermatol. 2001 Jun;144(6):1133-9
pubmed: 11422032
PLoS One. 2010 Dec 07;5(12):e15004
pubmed: 21165148
Cell Syst. 2015 Dec 23;1(6):417-425
pubmed: 26771021
N Engl J Med. 2012 Mar 29;366(13):1190-9
pubmed: 22455413
Drug Des Devel Ther. 2018 Nov 12;12:3879-3883
pubmed: 30518998
J Clin Invest. 2018 Oct 1;128(10):4669-4681
pubmed: 30198907
J Invest Dermatol. 2014 Jul;134(7):1828-1838
pubmed: 24441097
Proc Natl Acad Sci U S A. 2005 Oct 25;102(43):15545-50
pubmed: 16199517
Immunity. 2013 Jan 24;38(1):187-97
pubmed: 23260195
Nat Biotechnol. 2021 Jun;39(6):765-774
pubmed: 33462509
J Invest Dermatol. 2014 Dec;134(12):2898-2907
pubmed: 24945094
Bioinformatics. 2017 Apr 15;33(8):1179-1186
pubmed: 28088763
BMC Immunol. 2015 Feb 12;16:6
pubmed: 25879435
J Exp Med. 2015 Dec 14;212(13):2203-12
pubmed: 26621454
Nat Med. 2018 Jul;24(7):994-1004
pubmed: 29892065
Bioinformatics. 2013 Jan 1;29(1):15-21
pubmed: 23104886
Braz J Med Biol Res. 2016 Aug 15;49(9):e5374
pubmed: 27532281
Nature. 2017 Dec 14;552(7684):253-257
pubmed: 29211713
Cell. 2019 Jun 13;177(7):1888-1902.e21
pubmed: 31178118
J Invest Dermatol. 2013 Apr;133(4):973-9
pubmed: 23223131
J Immunol. 2003 Jan 1;170(1):477-86
pubmed: 12496434
J Am Acad Dermatol. 2017 Mar;76(3):418-431
pubmed: 28057361
Science. 2016 Apr 8;352(6282):189-96
pubmed: 27124452
J Immunol. 2014 Apr 1;192(7):3111-20
pubmed: 24610014
Front Immunol. 2018 Aug 02;9:1682
pubmed: 30127781
PLoS One. 2010 Nov 24;5(11):e14108
pubmed: 21124836
Sci Transl Med. 2010 Oct 6;2(52):52ra72
pubmed: 20926833
Am J Pathol. 2002 Jan;160(1):347-55
pubmed: 11786428