Two steps purification, biochemical characterization, thermodynamics and structure elucidation of thermostable alkaline serine protease from Nocardiopsis alba strain OM-5.
Actinobacteria
/ chemistry
Bacterial Proteins
/ chemistry
Detergents
Endopeptidases
/ chemistry
Enzyme Stability
/ physiology
Hydrogen-Ion Concentration
Kinetics
Nocardiopsis
/ enzymology
Serine
/ chemistry
Serine Endopeptidases
/ chemistry
Serine Proteases
/ isolation & purification
Surface-Active Agents
Temperature
Thermodynamics
Circular dichroism spectroscopy
Salt-tolerant Actinomycetes
Submerged cultivation
Thermodynamics
Thermostable protease
Journal
International journal of biological macromolecules
ISSN: 1879-0003
Titre abrégé: Int J Biol Macromol
Pays: Netherlands
ID NLM: 7909578
Informations de publication
Date de publication:
01 Feb 2021
01 Feb 2021
Historique:
received:
08
09
2020
revised:
26
11
2020
accepted:
07
12
2020
pubmed:
15
12
2020
medline:
20
4
2021
entrez:
14
12
2020
Statut:
ppublish
Résumé
The Nocardiopsis alba strain OM-5 showed maximum protease production in submerged culture. The OM-5 protease was purified by hydrophobic interaction chromatography. The purified protease of 68 kDa showed maximum activity (3312 ± 1.64 U/mL) at 70 °C and was quite stable at 80 °C up to 4 M NaCl (w/v) at pH 9. The purified protease showed significant activity and stability in different cations, denaturing agents, metal ions, and osmolytes. The thermodynamic parameters including deactivation rate constant (K
Identifiants
pubmed: 33316342
pii: S0141-8130(20)35219-3
doi: 10.1016/j.ijbiomac.2020.12.061
pii:
doi:
Substances chimiques
Bacterial Proteins
0
Detergents
0
Surface-Active Agents
0
Serine
452VLY9402
Endopeptidases
EC 3.4.-
Serine Proteases
EC 3.4.-
Serine Endopeptidases
EC 3.4.21.-
microbial serine proteinases
EC 3.4.21.62
alkaline protease
EC 3.4.99.-
Types de publication
Journal Article
Langues
eng
Sous-ensembles de citation
IM
Pagination
39-50Informations de copyright
Copyright © 2020 Elsevier B.V. All rights reserved.
Déclaration de conflit d'intérêts
Declaration of competing interest The authors declare that there are no conflicts of interest.