Mining the Unmapped Reads in Bovine RNA-Seq Data Reveals the Prevalence of Bovine Herpes Virus-6 in European Dairy Cows and the Associated Changes in Their Phenotype and Leucocyte Transcriptome.

BoHV-4 Gammaherpesvirinae ISGylation RNA-Seq bovine herpes virus-6 dairy cows endometritis interferon leukocyte mastitis

Journal

Viruses
ISSN: 1999-4915
Titre abrégé: Viruses
Pays: Switzerland
ID NLM: 101509722

Informations de publication

Date de publication:
16 12 2020
Historique:
received: 22 11 2020
revised: 08 12 2020
accepted: 09 12 2020
entrez: 19 12 2020
pubmed: 20 12 2020
medline: 12 1 2021
Statut: epublish

Résumé

Microbial RNA is detectable in host samples by aligning unmapped reads from RNA sequencing against taxon reference sequences, generating a score proportional to the microbial load. An RNA-Seq data analysis showed that 83.5% of leukocyte samples from six dairy herds in different EU countries contained bovine herpes virus-6 (BoHV-6). Phenotypic data on milk production, metabolic function, and disease collected during their first 50 days in milk (DIM) were compared between cows with low (1-200 and

Identifiants

pubmed: 33339352
pii: v12121451
doi: 10.3390/v12121451
pmc: PMC7768445
pii:
doi:

Types de publication

Journal Article Research Support, Non-U.S. Gov't

Langues

eng

Sous-ensembles de citation

IM

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Auteurs

Laura Buggiotti (L)

Department of Pathobiology and Population Sciences, Royal Veterinary College, Hawkshead Lane, Hatfield AL9 7TA, UK.

Zhangrui Cheng (Z)

Department of Pathobiology and Population Sciences, Royal Veterinary College, Hawkshead Lane, Hatfield AL9 7TA, UK.

D Claire Wathes (DC)

Department of Pathobiology and Population Sciences, Royal Veterinary College, Hawkshead Lane, Hatfield AL9 7TA, UK.
Department of Pathobiology and Population Sciences, Royal Veterinary College, Hawkshead Lane, Hatfield AL9 7TA, UK.

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