24-epibrassinolide-induced growth promotion of wheat seedlings is associated with changes in the proteome and tyrosine phosphoproteome.
24-epibrassinolide
Triticum aestivum L
protein tyrosine phosphorylation
proteome
Journal
Plant biology (Stuttgart, Germany)
ISSN: 1438-8677
Titre abrégé: Plant Biol (Stuttg)
Pays: England
ID NLM: 101148926
Informations de publication
Date de publication:
May 2021
May 2021
Historique:
received:
24
09
2020
accepted:
14
12
2020
pubmed:
29
12
2020
medline:
28
4
2021
entrez:
28
12
2020
Statut:
ppublish
Résumé
Brassinosteroids (BRs) represent a unique class of steroidal plant hormones that display pronounced growth-promoting activity at very low concentrations. Although many efforts have been made to characterize the molecular basis of BR action, little is known about the mechanisms behind the growth-promoting effect of BRs at protein level. Proteomic analysis of response to the steroid plant hormone 24-epibrassinolide (EBR) in wheat seedling shoots (Triticum aestivum L.) was performed using two-dimensional electrophoresis (2-DE) and immunoblotting with highly specific antibodies (PY20) to phosphotyrosine. EBR-modulated proteins and phosphotyrosine polypeptides were identified using MALDI-TOF mass spectrometry. The study revealed that EBR-stimulated growth of wheat seedlings was accompanied by changes in the content of multiple proteins as well as in tyrosine phosphorylation of numerous polypeptides. Among them, 22 differentially accumulated proteins and 13 phosphotyrosine proteins were identified. Based on their performed functions, the identified proteins are involved in physiological processes (photosynthesis, growth, energy and amino acid metabolism) closely associated with intensification of plant metabolism. The EBR-induced changes in protein abundance and tyrosine phosphorylation profile may contribute to growth stimulation of wheat seedlings under the action of EBR. The obtained data suggest an important role for EBR in the activation of protein metabolism underlying fundamental physiological processes, including growth promotion.
Substances chimiques
Brassinosteroids
0
Plant Proteins
0
Proteome
0
Steroids, Heterocyclic
0
Tyrosine
42HK56048U
brassinolide
Y9IQ1L53OX
Types de publication
Journal Article
Langues
eng
Sous-ensembles de citation
IM
Pagination
456-463Subventions
Organisme : Russian Foundation for Basic Research
ID : 20-04-00904а
Organisme : Russian Foundation for Basic Research
ID : 14-04-00731a
Organisme : State assignment of Russia
ID : AAAAA16-116020350029-1
Informations de copyright
© 2021 German Society for Plant Sciences and The Royal Botanical Society of the Netherlands.
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