Micropatterning of Cells on Gold Surfaces for Biophysical Applications.
Journal
STAR protocols
ISSN: 2666-1667
Titre abrégé: STAR Protoc
Pays: United States
ID NLM: 101769501
Informations de publication
Date de publication:
18 12 2020
18 12 2020
Historique:
entrez:
30
12
2020
pubmed:
31
12
2020
medline:
31
12
2020
Statut:
epublish
Résumé
We developed a reproducible micropatterning method to manipulate and normalize cell shape and cell-cell separation on gold. We used methoxy polyethylene glycol thiol (PEG-SH) to create a self-assembled monolayer that can be oxidized at desired shapes through a photomask with deep UV light. The oxidized PEG can be coated with extracellular matrix proteins and seeded with cells adopting the pre-defined shape. The developed and analyzed surfaces can be used in a wide range of biophysical applications.
Identifiants
pubmed: 33377002
doi: 10.1016/j.xpro.2020.100106
pii: S2666-1667(20)30093-9
pmc: PMC7756938
doi:
Substances chimiques
Sulfhydryl Compounds
0
Polyethylene Glycols
3WJQ0SDW1A
Gold
7440-57-5
monomethoxypolyethylene glycol
9004-74-4
Types de publication
Journal Article
Research Support, Non-U.S. Gov't
Langues
eng
Pagination
100106Informations de copyright
© 2020 The Authors.
Déclaration de conflit d'intérêts
The authors declare no competing interests.
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