Application of electrical treatment on Euglena gracilis for increasing paramylon production.
Electrical stimulation
Euglena gracilis
Paramylon
ddPCR and beta glucan synthases
Journal
Applied microbiology and biotechnology
ISSN: 1432-0614
Titre abrégé: Appl Microbiol Biotechnol
Pays: Germany
ID NLM: 8406612
Informations de publication
Date de publication:
Feb 2021
Feb 2021
Historique:
received:
16
04
2020
accepted:
23
11
2020
revised:
10
09
2020
pubmed:
9
1
2021
medline:
15
5
2021
entrez:
8
1
2021
Statut:
ppublish
Résumé
Paramylon also called β-1,3-glucan is a value-added product produced from Euglena gracilis. Recently, researchers have developed various strategies for the enhanced paramylon production, among which electrical treatment for microbial stimulation can be an alternative owing to the applicability to large-scale cultivation. In this study, we applied the electrical treatment for enhanced paramylon production and found the proper treatment conditions. Under the treatment with platinum electrodes at 10 mA, the paramylon production of treated cells was significantly increased about 2.5-fold, compared to those of the untreated cells, although the density of cells was maintained due to considerable stress. The size of treated cells became larger, possibly due to the increased level of paramylon production within the cells. Accordingly, the contents of glucose uptake, glucose-6-phosphate (G6P), glucose-1-phosphate (G1P), and uridine diphosphoglucose (UDPG) were shifted to appropriate states for the process of paramylon synthesis under the treatment. The increased level of transcripts encoding glucan synthase-like 2 (EgGSL2) was also confirmed via droplet digital PCR (ddPCR) under the treatment. Overall, this study makes a major contribution to research on electrical stimulation and provides new insights into E. gracilis metabolism like paramylon synthesis. KEY POINTS: • Electrical treatment induced the paramylon production and morphological change of Euglena gracilis. • The glucose uptake of E. gracilis was increased during the electrical treatment, fueling the paramylon synthesis.
Identifiants
pubmed: 33415369
doi: 10.1007/s00253-020-11033-9
pii: 10.1007/s00253-020-11033-9
doi:
Substances chimiques
Glucans
0
paramylon
51052-65-4
Uridine Diphosphate Glucose
V50K1D7P4Y
Types de publication
Journal Article
Langues
eng
Sous-ensembles de citation
IM
Pagination
1031-1039Subventions
Organisme : Ministry of Oceans and Fisheries
ID : 20170488
Organisme : National Research Foundation of Korea
ID : 2019R1A2C2087449
Organisme : National Natural Science Foundation of China
ID : 51878309
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