Transcriptomic analysis of early stages of intestinal regeneration in Holothuria glaberrima.
Journal
Scientific reports
ISSN: 2045-2322
Titre abrégé: Sci Rep
Pays: England
ID NLM: 101563288
Informations de publication
Date de publication:
11 01 2021
11 01 2021
Historique:
received:
13
09
2020
accepted:
04
12
2020
entrez:
12
1
2021
pubmed:
13
1
2021
medline:
11
8
2021
Statut:
epublish
Résumé
Echinoderms comprise a group of animals with impressive regenerative capabilities. They can replace complex internal organs following injury or autotomy. In holothurians or sea cucumbers, cellular processes of intestinal regeneration have been extensively studied. The molecular machinery behind this faculty, however, remains to be understood. Here we assembled and annotated a de novo transcriptome using RNA-seq data consisting of regenerating and non-regenerating intestinal tissues from the sea cucumber Holothuria glaberrima. Comparisons of differential expression were made using the mesentery as a reference against 24 h and 3 days regenerating intestine, revealing a large number of differentially expressed transcripts. Gene ontology and pathway enrichment analysis showed evidence of increasing transcriptional activity. Further analysis of transcripts associated with transcription factors revealed diverse expression patterns with mechanisms involving developmental and cancer-related activity that could be related to the regenerative process. Our study demonstrates the broad and diversified gene expression profile during the early stages of the process using the mesentery as the focal point of intestinal regeneration. It also establishes the genes that are the most important candidates in the cellular processes that underlie regenerative responses.
Identifiants
pubmed: 33431961
doi: 10.1038/s41598-020-79436-2
pii: 10.1038/s41598-020-79436-2
pmc: PMC7801731
doi:
Types de publication
Journal Article
Research Support, N.I.H., Extramural
Langues
eng
Sous-ensembles de citation
IM
Pagination
346Subventions
Organisme : NIGMS NIH HHS
ID : P20 GM103475
Pays : United States
Organisme : NIGMS NIH HHS
ID : R15 GM124595
Pays : United States
Organisme : NIH HHS
ID : R15NS01686
Pays : United States
Organisme : NIGMS NIH HHS
ID : P20GM103475
Pays : United States
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