The virulence of Salmonella Enteritidis in Galleria mellonella is improved by N-dodecanoyl-homoserine lactone.

Galleria mellonella N-Dodecanoyl-homoserine lactone Quorum sensing Salmonella Virulence

Journal

Microbial pathogenesis
ISSN: 1096-1208
Titre abrégé: Microb Pathog
Pays: England
ID NLM: 8606191

Informations de publication

Date de publication:
Mar 2021
Historique:
received: 07 08 2020
revised: 01 01 2021
accepted: 03 01 2021
pubmed: 15 1 2021
medline: 22 6 2021
entrez: 14 1 2021
Statut: ppublish

Résumé

Salmonella is a food and waterborne pathogen responsible for outbreaks worldwide, and it can survive during passage through the stomach and inside host phagocytic cells. Virulence genes are required for infection and survival in macrophages, and some are under the regulation of the quorum sensing (QS) system. This study investigated the influence of the autoinducer 1 (AI-1), N-dodecanoyl-homoserine lactone (C12-HSL), on the virulence of Salmonella PT4 using Galleria mellonella as an infection model. Salmonella PT4 was grown in the presence and absence of C12-HSL under anaerobic conditions for 7 h, and the expression of rpoS, arcA, arcB, and invA genes was evaluated. After the inoculation of G. mellonella with the median lethal dose (LD

Identifiants

pubmed: 33444697
pii: S0882-4010(21)00002-4
doi: 10.1016/j.micpath.2021.104730
pii:
doi:

Substances chimiques

homoserine lactone 1192-20-7
Homoserine 6KA95X0IVO
4-Butyrolactone OL659KIY4X

Types de publication

Journal Article

Langues

eng

Sous-ensembles de citation

IM

Pagination

104730

Informations de copyright

Copyright © 2021. Published by Elsevier Ltd.

Auteurs

Leonardo Luiz de Freitas (L)

Departmento de Microbiologia, Universidade Federal de Viçosa (UFV), Viçosa, Minas Gerais, Brazil.

Fernanda Pereira da Silva (F)

Departmento de Microbiologia, Universidade Federal de Viçosa (UFV), Viçosa, Minas Gerais, Brazil.

Kenner Morais Fernandes (KM)

Departamento de Biologia Geral, Universidade Federal de Viçosa (UFV), Viçosa, Minas Gerais, Brazil.

Deisy Guimarães Carneiro (DG)

Departmento de Microbiologia, Universidade Federal de Viçosa (UFV), Viçosa, Minas Gerais, Brazil.

Leandro Licursi de Oliveira (L)

Departamento de Biologia Geral, Universidade Federal de Viçosa (UFV), Viçosa, Minas Gerais, Brazil.

Gustavo Ferreira Martins (GF)

Departamento de Biologia Geral, Universidade Federal de Viçosa (UFV), Viçosa, Minas Gerais, Brazil.

Maria Cristina Dantas Vanetti (MC)

Departmento de Microbiologia, Universidade Federal de Viçosa (UFV), Viçosa, Minas Gerais, Brazil. Electronic address: mvanetti@ufv.br.

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Classifications MeSH