Degradation of complex arabinoxylans by human colonic Bacteroidetes.
Bacterial Proteins
/ genetics
Bacteroides
/ enzymology
Colon
/ microbiology
Coumaric Acids
/ metabolism
Crystallography, X-Ray
Dietary Fiber
/ metabolism
Enzyme Assays
Esterases
/ genetics
Gastrointestinal Microbiome
/ physiology
Humans
Intestinal Mucosa
/ microbiology
Molecular Dynamics Simulation
Multigene Family
/ genetics
Substrate Specificity
Xylans
/ chemistry
Journal
Nature communications
ISSN: 2041-1723
Titre abrégé: Nat Commun
Pays: England
ID NLM: 101528555
Informations de publication
Date de publication:
19 01 2021
19 01 2021
Historique:
received:
27
02
2019
accepted:
09
12
2020
entrez:
20
1
2021
pubmed:
21
1
2021
medline:
2
2
2021
Statut:
epublish
Résumé
Some Bacteroidetes and other human colonic bacteria can degrade arabinoxylans, common polysaccharides found in dietary fiber. Previous work has identified gene clusters (polysaccharide-utilization loci, PULs) for degradation of simple arabinoxylans. However, the degradation of complex arabinoxylans (containing side chains such as ferulic acid, a phenolic compound) is poorly understood. Here, we identify a PUL that encodes multiple esterases for degradation of complex arabinoxylans in Bacteroides species. The PUL is specifically upregulated in the presence of complex arabinoxylans. We characterize some of the esterases biochemically and structurally, and show that they release ferulic acid from complex arabinoxylans. Growth of four different colonic Bacteroidetes members, including Bacteroides intestinalis, on complex arabinoxylans results in accumulation of ferulic acid, a compound known to have antioxidative and immunomodulatory properties.
Identifiants
pubmed: 33469030
doi: 10.1038/s41467-020-20737-5
pii: 10.1038/s41467-020-20737-5
pmc: PMC7815789
doi:
Substances chimiques
Bacterial Proteins
0
Coumaric Acids
0
Dietary Fiber
0
Xylans
0
arabinoxylan
9040-27-1
ferulic acid
AVM951ZWST
Esterases
EC 3.1.-
Types de publication
Journal Article
Research Support, N.I.H., Extramural
Research Support, Non-U.S. Gov't
Langues
eng
Sous-ensembles de citation
IM
Pagination
459Subventions
Organisme : NIGMS NIH HHS
ID : R01 GM140306
Pays : United States
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