Whole genomic sequencing based genotyping reveals a specific X3 sublineage restricted to Mexico and related with multidrug resistance.
Adult
Antitubercular Agents
/ pharmacology
Cross-Sectional Studies
DNA, Bacterial
/ chemistry
Drug Resistance, Multiple, Bacterial
/ drug effects
Female
Genomics
/ methods
Genotype
Genotyping Techniques
/ methods
Humans
Male
Mexico
Middle Aged
Multigene Family
/ genetics
Mycobacterium tuberculosis
/ drug effects
Phylogeny
Polymorphism, Single Nucleotide
Tuberculosis, Multidrug-Resistant
/ drug therapy
Whole Genome Sequencing
/ methods
Journal
Scientific reports
ISSN: 2045-2322
Titre abrégé: Sci Rep
Pays: England
ID NLM: 101563288
Informations de publication
Date de publication:
21 01 2021
21 01 2021
Historique:
received:
21
07
2020
accepted:
08
12
2020
entrez:
22
1
2021
pubmed:
23
1
2021
medline:
21
9
2021
Statut:
epublish
Résumé
Whole genome sequencing (WGS) has been shown to be superior to traditional procedures of genotyping in tuberculosis (TB), nevertheless, reports of its use in drug resistant TB (DR-TB) isolates circulating in Mexico, are practically unknown. Considering the above the main of this work was to identify and characterize the lineages and genomic transmission clusters present in 67 DR-TB isolates circulating in southeastern Mexico. The results show the presence of three major lineages: L1 (3%), L2 (3%) and L4 (94%), the last one included 16 sublineages. Sublineage 4.1.1.3 (X3) was predominant in 18 (27%) of the isolates, including one genomic cluster, formed by eleven multidrug resistant isolates and sharing the SIT 3278, which seems to be restricted to Mexico. By the use of WGS, it was possible to identify the high prevalence of L4 and a high number of sublineages circulating in the region, also was recognized the presence of a novel X3 sublineage, formed exclusively by multidrug resistant isolates and with restrictive circulation in Mexico for at least the past 17 years.
Identifiants
pubmed: 33479318
doi: 10.1038/s41598-020-80919-5
pii: 10.1038/s41598-020-80919-5
pmc: PMC7820219
doi:
Substances chimiques
Antitubercular Agents
0
DNA, Bacterial
0
Types de publication
Journal Article
Research Support, Non-U.S. Gov't
Langues
eng
Sous-ensembles de citation
IM
Pagination
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