Lycopene β-cyclase expression influences plant physiology, development, and metabolism in tobacco plants.
Nicotiana tabacum cv. Petit Havana
RNAi
biomass
carotenoids
lycopene β-cyclase
photosynthesis
phytohormones
transplastomic
β-Carotene
Journal
Journal of experimental botany
ISSN: 1460-2431
Titre abrégé: J Exp Bot
Pays: England
ID NLM: 9882906
Informations de publication
Date de publication:
29 03 2021
29 03 2021
Historique:
received:
11
08
2020
accepted:
19
01
2021
pubmed:
24
1
2021
medline:
22
5
2021
entrez:
23
1
2021
Statut:
ppublish
Résumé
Carotenoids are important isoprenoids produced in the plastids of photosynthetic organisms that play key roles in photoprotection and antioxidative processes. β-Carotene is generated from lycopene by lycopene β-cyclase (LCYB). Previously, we demonstrated that the introduction of the Daucus carota (carrot) DcLCYB1 gene into tobacco (cv. Xanthi) resulted in increased levels of abscisic acid (ABA) and especially gibberellins (GAs), resulting in increased plant yield. In order to understand this phenomenon prior to exporting this genetic strategy to crops, we generated tobacco (Nicotiana tabacum cv. Petit Havana) mutants that exhibited a wide range of LCYB expression. Transplastomic plants expressing DcLCYB1 at high levels showed a wild-type-like growth, even though their pigment content was increased and their leaf GA1 content was reduced. RNA interference (RNAi) NtLCYB lines showed different reductions in NtLCYB transcript abundance, correlating with reduced pigment content and plant variegation. Photosynthesis (leaf absorptance, Fv/Fm, and light-saturated capacity of linear electron transport) and plant growth were impaired. Remarkably, drastic changes in phytohormone content also occurred in the RNAi lines. However, external application of phytohormones was not sufficient to rescue these phenotypes, suggesting that altered photosynthetic efficiency might be another important factor explaining their reduced biomass. These results show that LCYB expression influences plant biomass by different mechanisms and suggests thresholds for LCYB expression levels that might be beneficial or detrimental for plant growth.
Identifiants
pubmed: 33484250
pii: 6116658
doi: 10.1093/jxb/erab029
pmc: PMC8006556
doi:
Substances chimiques
Plant Proteins
0
Carotenoids
36-88-4
Intramolecular Lyases
EC 5.5.-
lycopene cyclase-isomerase
EC 5.5.-
Types de publication
Journal Article
Research Support, Non-U.S. Gov't
Langues
eng
Sous-ensembles de citation
IM
Pagination
2544-2569Informations de copyright
© The Author(s) 2021. Published by Oxford University Press on behalf of the Society for Experimental Biology.
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