Particle packed mixed-mode chromatographic stationary phase for the separation of peptide in liquid chromatography.
mixed-mode chromatography
peptide separation
polymerization reaction
reproducibility
silica monolith particles
Journal
Journal of separation science
ISSN: 1615-9314
Titre abrégé: J Sep Sci
Pays: Germany
ID NLM: 101088554
Informations de publication
Date de publication:
Apr 2021
Apr 2021
Historique:
revised:
20
01
2021
received:
01
01
2021
accepted:
21
01
2021
pubmed:
26
1
2021
medline:
26
10
2021
entrez:
25
1
2021
Statut:
ppublish
Résumé
A particle-based stationary phase has been prepared for the separation of five synthetic peptides and a mixture containing tryptic digest of cytochrome C in liquid chromatography. Particles originating from silica monolith were differentially sedimented to obtain 1-2 μm particles. A stationary phase was achieved by the coating of poly(styrene-methacrylic acid-N-phenylacrylamide) copolymer onto the particles via reversible addition-fragmentation chain transfer polymerization reaction. Stainless steel column (30 cm long and 1 mm internal diameter) was packed with stationary phase. Very high separation efficiency (ca. 351 000 plates/m) was achieved for five commercial peptides with a percent relative standard deviation of less than 1%. Protocol for the synthesis and modification of silica monolith particles has been well optimized with a good reproducibility both in particle and pore size. The column resolved about 21 peptide components from a mixture containing tryptic digest of cytochrome C, under the elution conditions of acetonitrile/15 mM ammonium format (65/35 v/v%) with a flow rate of 28 μL/min.
Identifiants
pubmed: 33492780
doi: 10.1002/jssc.202100001
doi:
Substances chimiques
Peptides
0
Cytochromes c
9007-43-6
Types de publication
Journal Article
Langues
eng
Sous-ensembles de citation
IM
Pagination
1430-1439Informations de copyright
© 2021 Wiley-VCH GmbH.
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