Stability and recovery issues concerning chondroitin sulfate disaccharide analysis.
Acetonitriles
/ chemistry
Chemistry Techniques, Analytical
Chondroitin Sulfates
/ chemistry
Chromatography, High Pressure Liquid
Chromatography, Liquid
Disaccharides
/ chemistry
Freeze Drying
Glycosaminoglycans
/ chemistry
Mass Spectrometry
Methanol
/ chemistry
Organic Chemicals
Solvents
/ chemistry
Chondroitin-sulfate
Glycosaminoglycan
High-performance liquid chromatography
Mass spectrometry
Recovery
Stability
Journal
Analytical and bioanalytical chemistry
ISSN: 1618-2650
Titre abrégé: Anal Bioanal Chem
Pays: Germany
ID NLM: 101134327
Informations de publication
Date de publication:
Mar 2021
Mar 2021
Historique:
received:
30
11
2020
accepted:
04
01
2021
pubmed:
29
1
2021
medline:
3
8
2021
entrez:
28
1
2021
Statut:
ppublish
Résumé
Chondroitin sulfate (CS) is a widely studied class of glycosaminoglycans, responsible for diverse biological functions. Structural analysis of CS is generally based on disaccharide analysis. Sample preparation is a key analytical issue in this case. However, a detailed study on the stability and recovery of CS-derived species has been lacking so far. We have found that for solvent exchange, in general, vacuum evaporation (SpeedVac) is much preferable than lyophilization. Moreover, in the case of aqueous solutions, higher recovery was experienced than in solutions with high organic solvent content. Storage of the resulting disaccharide mixture in typical HPLC injection solvents is also critical; decomposition starts after 12 h at 4 °C; therefore, the mixtures should not be kept in the sample tray of an automatic injector for a long time. The study, therefore, lays down suggestions on proper sample preparation and measurement conditions for biologically derived chondroitin sulfate species.
Identifiants
pubmed: 33506337
doi: 10.1007/s00216-021-03152-7
pii: 10.1007/s00216-021-03152-7
pmc: PMC7925331
doi:
Substances chimiques
Acetonitriles
0
Disaccharides
0
Glycosaminoglycans
0
Organic Chemicals
0
Solvents
0
Chondroitin Sulfates
9007-28-7
Methanol
Y4S76JWI15
acetonitrile
Z072SB282N
Types de publication
Journal Article
Langues
eng
Sous-ensembles de citation
IM
Pagination
1779-1785Subventions
Organisme : National Research, Development and Innovation Fund of Hungary
ID : 2018-1.2.1-NKP-2018-00005
Organisme : Magyar Tudományos Akadémia
ID : János Bolyai Research Scholarship
Organisme : National Research, Development and Innovation Office
ID : OTKA PD 121187
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