A scaffold lncRNA shapes the mitosis to meiosis switch.
Gene Expression Regulation, Fungal
Meiosis
/ genetics
Mitosis
/ genetics
Protein Binding
RNA Stability
/ genetics
RNA, Fungal
/ genetics
RNA, Long Noncoding
/ genetics
RNA, Messenger
/ genetics
RNA-Binding Proteins
/ genetics
Schizosaccharomyces
/ genetics
Schizosaccharomyces pombe Proteins
/ genetics
mRNA Cleavage and Polyadenylation Factors
/ genetics
Journal
Nature communications
ISSN: 2041-1723
Titre abrégé: Nat Commun
Pays: England
ID NLM: 101528555
Informations de publication
Date de publication:
03 02 2021
03 02 2021
Historique:
received:
15
07
2020
accepted:
05
01
2021
entrez:
4
2
2021
pubmed:
5
2
2021
medline:
23
2
2021
Statut:
epublish
Résumé
Long non-coding RNAs (lncRNAs) contribute to the regulation of gene expression in response to intra- or extracellular signals but the underlying molecular mechanisms remain largely unexplored. Here, we identify an uncharacterized lncRNA as a central player in shaping the meiotic gene expression program in fission yeast. We report that this regulatory RNA, termed mamRNA, scaffolds the antagonistic RNA-binding proteins Mmi1 and Mei2 to ensure their reciprocal inhibition and fine tune meiotic mRNA degradation during mitotic growth. Mechanistically, mamRNA allows Mmi1 to target Mei2 for ubiquitin-mediated downregulation, and conversely enables accumulating Mei2 to impede Mmi1 activity, thereby reinforcing the mitosis to meiosis switch. These regulations also occur within a unique Mmi1-containing nuclear body, positioning mamRNA as a spatially-confined sensor of Mei2 levels. Our results thus provide a mechanistic basis for the mutual control of gametogenesis effectors and further expand our vision of the regulatory potential of lncRNAs.
Identifiants
pubmed: 33536434
doi: 10.1038/s41467-021-21032-7
pii: 10.1038/s41467-021-21032-7
pmc: PMC7859202
doi:
Substances chimiques
Mmi1 protein, S pombe
0
RNA, Fungal
0
RNA, Long Noncoding
0
RNA, Messenger
0
RNA-Binding Proteins
0
Schizosaccharomyces pombe Proteins
0
mRNA Cleavage and Polyadenylation Factors
0
mei2 protein, S pombe
0
Types de publication
Journal Article
Research Support, Non-U.S. Gov't
Langues
eng
Sous-ensembles de citation
IM
Pagination
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