A staged representation electrochemical stimulated strategy to regulate intracellular reducing power for improving succinate production by Escherichia coli AFP111.
E. coli
NADH/NAD+
microbial electrolysis cell
oxidation reduction potential
succinate
Journal
Biotechnology journal
ISSN: 1860-7314
Titre abrégé: Biotechnol J
Pays: Germany
ID NLM: 101265833
Informations de publication
Date de publication:
May 2021
May 2021
Historique:
revised:
06
01
2021
received:
25
09
2020
accepted:
12
01
2021
pubmed:
14
2
2021
medline:
12
5
2021
entrez:
13
2
2021
Statut:
ppublish
Résumé
Escherichia coli AFP111 was previously engineered for succinate production by eliminating byproducts of synthesis pathways. Still, the succinate yield is limited due to the insufficient NADH supplement, when fed with glucose. Microbial electrolysis cell (MEC) allows microorganisms to perform unbalanced fermentation by establishing polarized cathode interaction. In this study, a cathode electrode was used as an additional electron donor to improve succinate synthesis by E. coli AFP111. In MEC with -0.65 V (vs. Ag/AgCl) poised on cathode electrode, 95.72% electrons were transferred into cells via neutral red (NR), and the ratio of NADH/NAD Staged representation of electrochemical stimulated strategy is effective for succinate producing in engineered E. coli by regulating intracellular reducing power, which provides a new concept for producing reduced metabolite in unbalanced fermentation.
Sections du résumé
BACKGROUND
BACKGROUND
Escherichia coli AFP111 was previously engineered for succinate production by eliminating byproducts of synthesis pathways. Still, the succinate yield is limited due to the insufficient NADH supplement, when fed with glucose. Microbial electrolysis cell (MEC) allows microorganisms to perform unbalanced fermentation by establishing polarized cathode interaction.
METHODS AND RESULTS
RESULTS
In this study, a cathode electrode was used as an additional electron donor to improve succinate synthesis by E. coli AFP111. In MEC with -0.65 V (vs. Ag/AgCl) poised on cathode electrode, 95.72% electrons were transferred into cells via neutral red (NR), and the ratio of NADH/NAD
CONCLUSION AND IMPLICATIONS
CONCLUSIONS
Staged representation of electrochemical stimulated strategy is effective for succinate producing in engineered E. coli by regulating intracellular reducing power, which provides a new concept for producing reduced metabolite in unbalanced fermentation.
Identifiants
pubmed: 33580738
doi: 10.1002/biot.202000415
doi:
Substances chimiques
Escherichia coli Proteins
0
Succinic Acid
AB6MNQ6J6L
Glucose
IY9XDZ35W2
Types de publication
Journal Article
Langues
eng
Sous-ensembles de citation
IM
Pagination
e2000415Subventions
Organisme : Jiangsu Synergetic Innovation Center for Advanced Bio-Manufacture of China
Organisme : National Natural Science Foundation of China
ID : 21706124
Organisme : National Natural Science Foundation of China
ID : 21727818
Organisme : National Key R&D Program of China
ID : 2018YFA0901500
Organisme : Key Science and Technology Project of Jiangsu Province
ID : BE2016389
Informations de copyright
© 2021 Wiley-VCH GmbH.
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