Quantitative Yeast-Yeast Two Hybrid for the Discovery and Binding Affinity Estimation of Protein-Protein Interactions.
cDNA library
post-translational modifications
protein engineering
protein−protein interactions
yeast surface display
yeast two-hybrid
Journal
ACS synthetic biology
ISSN: 2161-5063
Titre abrégé: ACS Synth Biol
Pays: United States
ID NLM: 101575075
Informations de publication
Date de publication:
19 03 2021
19 03 2021
Historique:
pubmed:
16
2
2021
medline:
3
11
2021
entrez:
15
2
2021
Statut:
ppublish
Résumé
Quantifying the binding affinity of protein-protein interactions is important for elucidating connections within biochemical signaling pathways, as well as characterization of binding proteins isolated from combinatorial libraries. We describe a quantitative yeast-yeast two-hybrid (qYY2H) system that not only enables the discovery of specific protein-protein interactions but also efficient, quantitative estimation of their binding affinities (
Identifiants
pubmed: 33587591
doi: 10.1021/acssynbio.0c00472
doi:
Substances chimiques
Smad3 Protein
0
Transcription Factors
0
Types de publication
Journal Article
Research Support, N.I.H., Extramural
Research Support, U.S. Gov't, Non-P.H.S.
Langues
eng
Sous-ensembles de citation
IM
Pagination
505-514Subventions
Organisme : NIGMS NIH HHS
ID : T32 GM008776
Pays : United States