Traceless Staudinger Ligation for Bioconjugation of RNA.
Journal
Current protocols
ISSN: 2691-1299
Titre abrégé: Curr Protoc
Pays: United States
ID NLM: 101773894
Informations de publication
Date de publication:
Feb 2021
Feb 2021
Historique:
entrez:
16
2
2021
pubmed:
17
2
2021
medline:
22
6
2021
Statut:
ppublish
Résumé
Staudinger ligation is an attractive bioorthogonal reaction for use in studying biomolecules due to its capacity to form a native amide bond between a tag and a biomolecule. Here, we explore the traceless variant of the Staudinger ligation for 3'-end modification of oligoribonucleotides. The procedure involves (i) synthesis of phosphine-containing reactive groups, affinity purification tags, or photoactivatable benzophenone probe, (ii) synthesis of 2'-azido dinucleotides and 24-nt RNA, and (iii) traceless Staudinger ligation experiments. Each phosphine was characterized by
Substances chimiques
Amides
0
Azides
0
RNA
63231-63-0
Types de publication
Journal Article
Langues
eng
Sous-ensembles de citation
IM
Pagination
e42Subventions
Organisme : Agence National de la Recherche (ANR)
ID : ANR-17-CE07-0041-02
Commentaires et corrections
Type : ErratumIn
Type : ErratumIn
Informations de copyright
© 2021 Wiley Periodicals LLC.
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