Liposomes as Carriers of Membrane-Associated Proteins and Peptides for Mass Spectrometric Analysis.
liposomes
mass spectrometry
membrane mimetics
peripheral membrane proteins
protein-lipid interactions
Journal
Angewandte Chemie (International ed. in English)
ISSN: 1521-3773
Titre abrégé: Angew Chem Int Ed Engl
Pays: Germany
ID NLM: 0370543
Informations de publication
Date de publication:
10 05 2021
10 05 2021
Historique:
received:
26
01
2021
pubmed:
19
2
2021
medline:
10
8
2021
entrez:
18
2
2021
Statut:
ppublish
Résumé
Membrane proteins are key players of the cell. Their structure and the interactions they form with their lipid environment are required to understand their function. Here we explore liposomes as membrane mimetics for mass spectrometric analysis of peripheral membrane proteins and peptides. Liposomes are advantageous over other membrane mimetics in that they are easy to prepare, can be varied in size and composition, and are suitable for functional assays. We demonstrate that they dissociate into lipid clusters in the gas phase of a mass spectrometer while intact protein and protein-lipid complexes are retained. We exemplify this approach by employing different liposomes including proteoliposomes of two model peptides/proteins differing in size. Our results pave the way for the general application of liposomes for mass spectrometric analysis of membrane-associated proteins.
Identifiants
pubmed: 33599387
doi: 10.1002/anie.202101242
pmc: PMC8252038
doi:
Substances chimiques
Liposomes
0
Membrane Proteins
0
Peptides
0
Types de publication
Journal Article
Research Support, Non-U.S. Gov't
Langues
eng
Sous-ensembles de citation
IM
Pagination
11523-11530Informations de copyright
© 2021 The Authors. Angewandte Chemie International Edition published by Wiley-VCH GmbH.
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