Generation of two gene edited iPSC-lines carrying a DOX-inducible NGN2 expression cassette with and without GFP in the AAVS1 locus.

Cell Differentiation Gene Editing Genes, Reporter Induced Pluripotent Stem Cells Transgenes

Journal

Stem cell research
ISSN: 1876-7753
Titre abrégé: Stem Cell Res
Pays: England
ID NLM: 101316957

Informations de publication

Date de publication:
04 2021
Historique:
received: 03 12 2020
revised: 20 01 2021
accepted: 05 02 2021
pubmed: 21 2 2021
medline: 1 7 2021
entrez: 20 2 2021
Statut: ppublish

Résumé

Neurog2 is the gene encoding the neuronal transcription factor NGN2, which can convert stem cells into functional neurons in a fast and efficient way. Here we report the generation of two iPS cell lines, where DOX inducible constructs of neurog2 either without or with T2A-eGFP were inserted into the safe-site locus AAVS1. These iPS cell lines, BIONi010-C-13 and BIONi010-C-15, respectively, stay pluripotent without DOX but differentiate to (GFP positive) neurons when DOX is added without the need of differentiation factors.

Identifiants

DOI: 10.1016/j.scr.2021.102240 PMID: 33610017
pubmed: 33610017
pii: S1873-5061(21)00086-6
doi: 10.1016/j.scr.2021.102240
pii:
doi:

Types de publication

Journal Article Research Support, Non-U.S. Gov't

Langues

eng

Sous-ensembles de citation

IM

Pagination

102240

Informations de copyright

Copyright © 2021 Janssen Pharmaceutica. Published by Elsevier B.V. All rights reserved.

Auteurs

Benjamin Schmid (B)

Bioneer A/S, Kogle Alle 2, 2970 Hørsholm, Denmark.

Bjørn Holst (B)

Bioneer A/S, Kogle Alle 2, 2970 Hørsholm, Denmark.

Ulla Poulsen (U)

Bioneer A/S, Kogle Alle 2, 2970 Hørsholm, Denmark.

Ida Jørring (I)

Bioneer A/S, Kogle Alle 2, 2970 Hørsholm, Denmark.

Christian Clausen (C)

Bioneer A/S, Kogle Alle 2, 2970 Hørsholm, Denmark.

M Rasmussen (M)

Bioneer A/S, Kogle Alle 2, 2970 Hørsholm, Denmark.

Ulrike A Mau-Holzmann (UA)

Institute of Medical Genetics and Applied Genomics, Division of Cytogenetics, Calwerstrasse 7, University of Tübingen, 72076, Germany.

Rachel Steeg (R)

Fraunhofer UK Research Ltd, Glasgow, UK.

Hugh Nuthall (H)

Eli Lilly & Company Limited, Bracknell, UK.

A Ebneth (A)

Neuroscience Therapeutic Area, Janssen Research & Development, A Division of Janssen Pharmaceutica NV, UK.

A Cabrera-Socorro (A)

Neuroscience Therapeutic Area, Janssen Research & Development, A Division of Janssen Pharmaceutica NV, UK. Electronic address: acabrer8@its.jnj.com.

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Classifications MeSH

questionsmedicales.fr › Phénomènes physiologiques cellulaires › Différenciation cellulaire › Generation of two gene edited iPSC-lines...
questionsmedicales.fr › Techniques d'investigation › Techniques génétiques › Génie génétique › Édition de gène › Generation of two gene edited iPSC-lines...
questionsmedicales.fr › Phénomènes génétiques › Structures génétiques › Génome › Composants de génome › Gènes › Gènes rapporteurs › Generation of two gene edited iPSC-lines...
questionsmedicales.fr › Cellules › Cellules souches › Cellules souches pluripotentes › Cellules souches pluripotentes induites › Generation of two gene edited iPSC-lines...
questionsmedicales.fr › Phénomènes génétiques › Structures génétiques › Génome › Composants de génome › Gènes › Transgènes › Generation of two gene edited iPSC-lines...