Rice calcium/calmodulin-dependent protein kinase directly phosphorylates a mitogen-activated protein kinase kinase to regulate abscisic acid responses.
Abscisic Acid
/ metabolism
Calcium
/ metabolism
Calcium-Calmodulin-Dependent Protein Kinases
/ metabolism
Mitogen-Activated Protein Kinase Kinases
/ chemistry
Models, Biological
Oryza
/ drug effects
Oxidative Stress
/ drug effects
Phosphorylation
/ drug effects
Phosphothreonine
/ metabolism
Plant Proteins
/ metabolism
Protein Binding
/ drug effects
Signal Transduction
/ drug effects
Stress, Physiological
/ drug effects
Water
Journal
The Plant cell
ISSN: 1532-298X
Titre abrégé: Plant Cell
Pays: England
ID NLM: 9208688
Informations de publication
Date de publication:
02 07 2021
02 07 2021
Historique:
received:
07
01
2021
accepted:
15
02
2021
pubmed:
26
2
2021
medline:
2
9
2021
entrez:
25
2
2021
Statut:
ppublish
Résumé
Calcium (Ca2+)/calmodulin (CaM)-dependent protein kinase (CCaMK) is an important positive regulator of abscisic acid (ABA) and abiotic stress signaling in plants and is believed to act upstream of mitogen-activated protein kinase (MAPK) in ABA signaling. However, it is unclear how CCaMK activates MAPK in ABA signaling. Here, we show that OsDMI3, a rice (Oryza sativa) CCaMK, directly interacts with and phosphorylates OsMKK1, a MAPK kinase (MKK) in rice, in vitro and in vivo. OsDMI3 was found to directly phosphorylate Thr-25 in the N-terminus of OsMKK1, and this Thr-25 phosphorylation is OsDMI3-specific in ABA signaling. The activation of OsMKK1 and its downstream kinase OsMPK1 is dependent on Thr-25 phosphorylation of OsMKK1 in ABA signaling. Moreover, ABA treatment induces phosphorylation in the activation loop of OsMKK1, and the two phosphorylations, in the N-terminus and in the activation loop, are independent. Further analyses revealed that OsDMI3-mediated phosphorylation of OsMKK1 positively regulates ABA responses in seed germination, root growth, and tolerance to both water stress and oxidative stress. Our results indicate that OsMKK1 is a direct target of OsDMI3, and OsDMI3-mediated phosphorylation of OsMKK1 plays an important role in activating the MAPK cascade and ABA signaling.
Identifiants
pubmed: 33630095
pii: 6149486
doi: 10.1093/plcell/koab071
pmc: PMC8254507
doi:
Substances chimiques
Plant Proteins
0
Water
059QF0KO0R
Phosphothreonine
1114-81-4
Abscisic Acid
72S9A8J5GW
Calcium-Calmodulin-Dependent Protein Kinases
EC 2.7.11.17
Mitogen-Activated Protein Kinase Kinases
EC 2.7.12.2
Calcium
SY7Q814VUP
Types de publication
Journal Article
Research Support, Non-U.S. Gov't
Langues
eng
Sous-ensembles de citation
IM
Pagination
1790-1812Informations de copyright
© American Society of Plant Biologists 2021. All rights reserved. For permissions, please email: journals.permissions@oup.com.
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