ASimulatoR: splice-aware RNA-Seq data simulation.


Journal

Bioinformatics (Oxford, England)
ISSN: 1367-4811
Titre abrégé: Bioinformatics
Pays: England
ID NLM: 9808944

Informations de publication

Date de publication:
29 09 2021
Historique:
received: 29 09 2020
revised: 16 02 2021
accepted: 25 02 2021
pubmed: 2 3 2021
medline: 2 2 2023
entrez: 1 3 2021
Statut: ppublish

Résumé

A plethora of tools exist for RNA-Seq data analysis with a focus on alternative splicing (AS). However, appropriate data for their comparative evaluation is missing. The R package ASimulatoR simulates gold standard RNA-Seq datasets with fine-grained control over the distribution of AS events, which allow for evaluating alternative splicing tools, e.g. to study the effect of sequencing depth on the performance of AS event detection. ASimulatoR is freely available at https://github.com/biomedbigdata/ASimulatoR as an R package under GPL-3 license.

Identifiants

pubmed: 33647976
pii: 6153968
doi: 10.1093/bioinformatics/btab142
doi:

Types de publication

Journal Article Research Support, Non-U.S. Gov't

Langues

eng

Sous-ensembles de citation

IM

Pagination

3008-3010

Subventions

Organisme : European Union's Horizon 2020 research and innovation programme
ID : 777111
Organisme : German Federal Ministry of Education and Research
ID : 01ZX1908A
Organisme : Deutsche Forschungsgemeinschaft
ID : 395357507

Informations de copyright

© The Author(s) 2021. Published by Oxford University Press. All rights reserved. For permissions, please e-mail: journals.permissions@oup.com.

Auteurs

Quirin Manz (Q)

Chair of Experimental Bioinformatics, TUM School of Life Sciences Weihenstephan, Technical University of Munich, 85354 Freising, Germany.

Olga Tsoy (O)

Chair of Experimental Bioinformatics, TUM School of Life Sciences Weihenstephan, Technical University of Munich, 85354 Freising, Germany.

Amit Fenn (A)

Chair of Experimental Bioinformatics, TUM School of Life Sciences Weihenstephan, Technical University of Munich, 85354 Freising, Germany.

Jan Baumbach (J)

Chair of Experimental Bioinformatics, TUM School of Life Sciences Weihenstephan, Technical University of Munich, 85354 Freising, Germany.
Department of Mathematics and Computer Science, University of Southern Denmark, 5230 Odense, Denmark.
Chair of Computational Systems Biology, University of Hamburg, 22607 Hamburg, Germany.

Uwe Völker (U)

Interfaculty Institute for Genetics and Functional Genomics, University Medicine Greifswald, 17475 Greifswald, Germany.

Markus List (M)

Chair of Experimental Bioinformatics, TUM School of Life Sciences Weihenstephan, Technical University of Munich, 85354 Freising, Germany.

Tim Kacprowski (T)

Chair of Experimental Bioinformatics, TUM School of Life Sciences Weihenstephan, Technical University of Munich, 85354 Freising, Germany.
Division Data Science in Biomedicine, Peter L. Reichertz Institute for Medical Informatics, TU Braunschweig and Hannover Medical School, 38106 Brunswick, Germany.
Braunschweig Integrated Centre of Systems Biology (BRICS), 38106 Braunschweig, Germany.

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Classifications MeSH