Adaptive evolution of engineered yeast for squalene production improvement and its genome-wide analysis.
Nile red
Saccharomyces cerevisiae
adaptive evolution
lipid droplet
squalene
whole-genome sequencing
Journal
Yeast (Chichester, England)
ISSN: 1097-0061
Titre abrégé: Yeast
Pays: England
ID NLM: 8607637
Informations de publication
Date de publication:
07 2021
07 2021
Historique:
revised:
22
02
2021
received:
18
05
2020
accepted:
24
02
2021
pubmed:
2
3
2021
medline:
26
10
2021
entrez:
1
3
2021
Statut:
ppublish
Résumé
In the present study, the adaptive evolution of a metabolically engineered Saccharomyces cerevisiae strain in the presence of an enzyme inhibitor terbinafine for enhanced squalene accumulation via serial transfer leads to the development of robust strains. After adaptation for nearly 1500 h, a strain with higher squalene production efficiency was identified at a specific growth rate of 0.28 h
Substances chimiques
Saccharomyces cerevisiae Proteins
0
Squalene
7QWM220FJH
Types de publication
Journal Article
Research Support, Non-U.S. Gov't
Langues
eng
Sous-ensembles de citation
IM
Pagination
424-437Informations de copyright
© 2021 John Wiley & Sons, Ltd.
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