RNA helicase DDX5 acts as a critical regulator for survival of neonatal mouse gonocytes.
Animals
Animals, Newborn
DEAD-box RNA Helicases
/ genetics
Gene Expression Regulation, Developmental
Genotype
Germ Cells
/ cytology
Glial Cell Line-Derived Neurotrophic Factor
/ metabolism
Infertility
/ metabolism
Male
Mice
Mice, Knockout
Sequence Analysis, RNA
Single-Cell Analysis
Testis
/ metabolism
DDX5
RNA-binding protein
gonocyte
spermatogonial stem cell
testis
Journal
Cell proliferation
ISSN: 1365-2184
Titre abrégé: Cell Prolif
Pays: England
ID NLM: 9105195
Informations de publication
Date de publication:
May 2021
May 2021
Historique:
revised:
11
01
2021
received:
17
11
2020
accepted:
14
01
2021
pubmed:
6
3
2021
medline:
12
5
2021
entrez:
5
3
2021
Statut:
ppublish
Résumé
Mammalian spermatogenesis is a biological process of male gamete formation. Gonocytes are the only precursors of spermatogonial stem cells (SSCs) which develop into mature spermatozoa. DDX5 is one of DEAD-box RNA helicases and expresses in male germ cells, suggesting that Ddx5 plays important functions during spermatogenesis. Here, we explore the functions of Ddx5 in regulating the specification of gonocytes. Germ cell-specific Ddx5 knockout (Ddx5 In male mice, Ddx5 was expressed in germ cells at different stages of development. Germ cell-specific Ddx5 knockout adult male mice were sterile due to completely devoid of germ cells. Male germ cells gradually disappeared in Ddx5 Our study reveals the critical roles of Ddx5 in fate determination of gonocytes, offering a novel insight into the pathogenesis of male sterility.
Identifiants
pubmed: 33666296
doi: 10.1111/cpr.13000
pmc: PMC8088469
doi:
Substances chimiques
Glial Cell Line-Derived Neurotrophic Factor
0
Ddx5 protein, mouse
EC 3.6.1.-
DEAD-box RNA Helicases
EC 3.6.4.13
Types de publication
Journal Article
Langues
eng
Sous-ensembles de citation
IM
Pagination
e13000Subventions
Organisme : The Ministry of Science and Technology of the People's Republic of China
ID : 2016YFA0100300
Organisme : Strategic Priority Research Program of the Chinese Academy of Sciences
ID : XDA16010502
Organisme : Strategic Priority Research Program of the Chinese Academy of Sciences
ID : XDA16020404
Organisme : The Ministry of Science and Technology of the People's Republic of China
ID : 2016YFA0100400
Organisme : National Natural Science Foundation of China
ID : 31925009
Organisme : National Natural Science Foundation of China
ID : 81902885
Organisme : National Natural Science Foundation of China
ID : 32000424
Organisme : National Natural Science Foundation of China
ID : 31871456
Organisme : Key Research & Development Program of Guangzhou Regenerative Medicine and Health Guangdong Laboratory
ID : 2018GZR110104007
Organisme : Science and Technology Planning Project of Guangdong Province, China
ID : 2019B020234004
Organisme : Science and Technology Planning Project of Guangdong Province, China
ID : 2020B1212060052
Organisme : Science and Technology Program of Guangzhou, China
ID : 201906010096
Organisme : Guangdong Basic and Applied Basic Research Foundation
ID : 2019A1515110028
Organisme : Guangdong Basic and Applied Basic Research Foundation
ID : 2019B151502054
Informations de copyright
© 2021 The Authors. Cell Proliferation Published by John Wiley & Sons Ltd.
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