Mitochondrial citrate synthase plays important roles in anthocyanin synthesis in petunia.

Anthocyanins / biosynthesis Citrate (si)-Synthase / genetics Flowers / enzymology Gene Expression Regulation, Plant Genes, Mitochondrial Petunia / enzymology
Anthocyanin synthesis Citrate synthase Flower development Leaf development Petunia

Journal

Plant science : an international journal of experimental plant biology
ISSN: 1873-2259
Titre abrégé: Plant Sci
Pays: Ireland
ID NLM: 9882015

Informations de publication

Date de publication:
Apr 2021
Historique:
received: 21 10 2020
revised: 26 01 2021
accepted: 30 01 2021
entrez: 11 3 2021
pubmed: 12 3 2021
medline: 2 6 2021
Statut: ppublish

Résumé

Anthocyanins are important flavonoid pigments in plants. Malonyl CoA is an important intermediate in anthocyanin synthesis, and citrate, formed by citrate synthase (CS) catalysing oxaloacetate, is the precursor for the formation of malonyl-CoA. CS is composed of two isoforms, mitochondrial citrate synthase (mCS), a key enzyme of the tricarboxylic acid (TCA) cycle, and citrate synthase (CSY) localizated in microbodies in plants. However, no CS isoform involvement in anthocyanin synthesis has been reported. In this study, we identified the entire CS family in petunia (Petunia hybrida): PhmCS, PhCSY1 and PhCSY2. We obtained petunia plants silenced for the three genes. PhmCS silencing resulted in abnormal development of leaves and flowers. The contents of citrate and anthocyanins were significantly reduced in flowers in PhmCS-silenced plants. However, silencing of PhCSY1 and/or PhCSY2 did not cause a visible phenotype change in petunia. These results showed that PhmCS is involved in anthocyanin synthesis and the development of leaves and flowers, and that the citrate involved in anthocyanin synthesis mainly derived from mitochondria rather than microbodies in petunia.

Identifiants

DOI: 10.1016/j.plantsci.2021.110835 PMID: 33691969
pubmed: 33691969
pii: S0168-9452(21)00024-8
doi: 10.1016/j.plantsci.2021.110835
pii:
doi:

Substances chimiques

Anthocyanins 0
Citrate (si)-Synthase EC 2.3.3.1

Types de publication

Journal Article

Langues

eng

Sous-ensembles de citation

IM

Pagination

110835

Informations de copyright

Copyright © 2021 Elsevier B.V. All rights reserved.

Auteurs

Huina Zhao (H)

Guangdong Key Laboratory for Innovative Development and Utilization of Forest Plant Germplasm, College of Forestry and Landscape Architecture, South China Agricultural University, Guangzhou 510642, China; College of Horticulture, South China Agricultural University, Guangzhou 510642, China; Lingnan Guangdong Laboratory of Modern Agriculture, Guangzhou, 510642, China. Electronic address: 837175641@qq.com.

Guoju Chen (G)

College of Horticulture, South China Agricultural University, Guangzhou 510642, China. Electronic address: gjchen@scau.edu.cn.

Lina Sang (L)

College of Horticulture, South China Agricultural University, Guangzhou 510642, China. Electronic address: 1277805553@qq.com.

Ying Deng (Y)

Guangdong Key Laboratory for Innovative Development and Utilization of Forest Plant Germplasm, College of Forestry and Landscape Architecture, South China Agricultural University, Guangzhou 510642, China. Electronic address: 1285455810@qq.com.

Lili Gao (L)

Guangdong Key Laboratory for Innovative Development and Utilization of Forest Plant Germplasm, College of Forestry and Landscape Architecture, South China Agricultural University, Guangzhou 510642, China. Electronic address: liligao16@163.com.

Yixun Yu (Y)

Guangdong Key Laboratory for Innovative Development and Utilization of Forest Plant Germplasm, College of Forestry and Landscape Architecture, South China Agricultural University, Guangzhou 510642, China; Lingnan Guangdong Laboratory of Modern Agriculture, Guangzhou, 510642, China. Electronic address: yuyixun@scau.edu.cn.

Juanxu Liu (J)

Guangdong Key Laboratory for Innovative Development and Utilization of Forest Plant Germplasm, College of Forestry and Landscape Architecture, South China Agricultural University, Guangzhou 510642, China. Electronic address: juanxuliu@scau.edu.cn.

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Classifications MeSH

questionsmedicales.fr › Facteurs biologiques › Pigments biologiques › Anthocyanes › Mitochondrial citrate synthase plays important...
questionsmedicales.fr › Enzymes et coenzymes › Enzymes › Transferases › Acyltransferases › Citrate (si)-synthase › Mitochondrial citrate synthase plays important...
questionsmedicales.fr › Structures de plante › Parties aériennes de plante › Sommités fleuries › Fleurs › Mitochondrial citrate synthase plays important...
questionsmedicales.fr › Phénomènes génétiques › Régulation de l'expression des gènes › Régulation de l'expression des gènes végétaux › Mitochondrial citrate synthase plays important...
questionsmedicales.fr › Phénomènes génétiques › Modes de transmission héréditaire › Hérédité extrachromosomique › Gènes de mitochondrie › Mitochondrial citrate synthase plays important...
questionsmedicales.fr › Eucaryotes › Viridiplantae › Streptophyta › Embryophyta › Tracheobionta › Magnoliopsida › Solanales › Solanaceae › Petunia › Mitochondrial citrate synthase plays important...