Sortase-assembled pili in
Gram positive
integrative structural biology
lysine isopeptide bond
pili
sortase
Journal
Proceedings of the National Academy of Sciences of the United States of America
ISSN: 1091-6490
Titre abrégé: Proc Natl Acad Sci U S A
Pays: United States
ID NLM: 7505876
Informations de publication
Date de publication:
23 03 2021
23 03 2021
Historique:
entrez:
16
3
2021
pubmed:
17
3
2021
medline:
7
9
2021
Statut:
ppublish
Résumé
Gram-positive bacteria assemble pili (fimbriae) on their surfaces to adhere to host tissues and to promote polymicrobial interactions. These hair-like structures, although very thin (1 to 5 nm), exhibit impressive tensile strengths because their protein components (pilins) are covalently crosslinked together via lysine-isopeptide bonds by pilus-specific sortase enzymes. While atomic structures of isolated pilins have been determined, how they are joined together by sortases and how these interpilin crosslinks stabilize pilus structure are poorly understood. Using a reconstituted pilus assembly system and hybrid structural biology methods, we elucidated the solution structure and dynamics of the crosslinked interface that is repeated to build the prototypical SpaA pilus from
Identifiants
pubmed: 33723052
pii: 2019649118
doi: 10.1073/pnas.2019649118
pmc: PMC7999877
pii:
doi:
Substances chimiques
Bacterial Proteins
0
Fimbriae Proteins
147680-16-8
Aminoacyltransferases
EC 2.3.2.-
sortase A
EC 2.3.2.-
Cysteine Endopeptidases
EC 3.4.22.-
Lysine
K3Z4F929H6
Types de publication
Journal Article
Research Support, N.I.H., Extramural
Research Support, Non-U.S. Gov't
Research Support, U.S. Gov't, Non-P.H.S.
Langues
eng
Sous-ensembles de citation
IM
Subventions
Organisme : NIGMS NIH HHS
ID : P30 GM124169
Pays : United States
Organisme : NIH HHS
ID : S10 OD025073
Pays : United States
Organisme : NIDCR NIH HHS
ID : R01 DE025015
Pays : United States
Organisme : NIH HHS
ID : S10 OD018483
Pays : United States
Organisme : NIAID NIH HHS
ID : R01 AI052217
Pays : United States
Organisme : NIGMS NIH HHS
ID : T32 GM007185
Pays : United States
Organisme : NIDCR NIH HHS
ID : R01 DE017382
Pays : United States
Organisme : NIH HHS
ID : S10 OD016336
Pays : United States
Déclaration de conflit d'intérêts
The authors declare no competing interest.
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