A microfluidic colorimetric biosensor for in-field detection of Salmonella in fresh-cut vegetables using thiolated polystyrene microspheres, hose-based microvalve and smartphone imaging APP.
Colorimetric
Foodborne pathogen
Fresh-cut vegetable
Microfluidic chip
Microvalve
Polystyrene microsphere
Smartphone
Journal
Food chemistry
ISSN: 1873-7072
Titre abrégé: Food Chem
Pays: England
ID NLM: 7702639
Informations de publication
Date de publication:
30 Aug 2021
30 Aug 2021
Historique:
received:
12
10
2020
revised:
25
02
2021
accepted:
05
03
2021
pubmed:
24
3
2021
medline:
7
5
2021
entrez:
23
3
2021
Statut:
ppublish
Résumé
A microfluidic colorimetric biosensor was developed using thiolated polystyrene microspheres (SH-PSs) for aggregating of gold nanoparticles (AuNPs), a novel hose-based microvalve for controlling the flow direction, and a smartphone imaging APP for monitoring colorimetric signals. Aptamer-PS-cysteamine conjugates were used as detection probes and reacted with Salmonella in samples. Complementary DNA - magnetic nanoparticle (cDNA - MNP) conjugates were used as capture probes, reacted with the free aptamer-PS-cysteamine conjugates. AuNPs were aggregated on the surface of Salmonella-aptamer-PS-cysteamine conjugates, resulting in a visible color change in the detection chamber, which indicating different concentrations of Salmonella. The limit of detection was low to 6.0 × 10
Identifiants
pubmed: 33756331
pii: S0308-8146(21)00584-7
doi: 10.1016/j.foodchem.2021.129578
pii:
doi:
Substances chimiques
Polystyrenes
0
Gold
7440-57-5
Types de publication
Journal Article
Langues
eng
Sous-ensembles de citation
IM
Pagination
129578Informations de copyright
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