Cell-type identity of the avian cochlea.

Animals Biomarkers / metabolism Chickens / anatomy & histology Cochlea / cytology Epithelium / physiology Gene Expression Profiling Gene Expression Regulation Hair Cells, Auditory, Inner / cytology Labyrinth Supporting Cells / cytology RNA-Seq Reproducibility of Results Single-Cell Analysis
Auditory periphery hair cell regeneration hearing inner ear otic scRNA-seq supporting cell

Journal

Cell reports
ISSN: 2211-1247
Titre abrégé: Cell Rep
Pays: United States
ID NLM: 101573691

Informations de publication

Date de publication:
23 03 2021
Historique:
received: 14 10 2020
revised: 22 01 2021
accepted: 03 03 2021
entrez: 24 3 2021
pubmed: 25 3 2021
medline: 28 1 2022
Statut: ppublish

Résumé

In contrast to mammals, birds recover naturally from acquired hearing loss, which makes them an ideal model for inner ear regeneration research. Here, we present a validated single-cell RNA sequencing resource of the avian cochlea. We describe specific markers for three distinct types of sensory hair cells, including a previously unknown subgroup, which we call superior tall hair cells. We identify markers for the supporting cells associated with tall hair cells, which represent the facultative stem cells of the avian inner ear. Likewise, we present markers for supporting cells that are located below the short cochlear hair cells. We further infer spatial expression gradients of hair cell genes along the tonotopic axis of the cochlea. This resource advances neurobiology, comparative biology, and regenerative medicine by providing a basis for comparative studies with non-regenerating mammalian cochleae and for longitudinal studies of the regenerating avian cochlea.

Identifiants

DOI: 10.1016/j.celrep.2021.108900 PMID: 33761346
pubmed: 33761346
pii: S2211-1247(21)00214-X
doi: 10.1016/j.celrep.2021.108900
pii:
doi:

Substances chimiques

Biomarkers 0

Types de publication

Journal Article Research Support, Non-U.S. Gov't

Langues

eng

Sous-ensembles de citation

IM

Pagination

108900

Commentaires et corrections

Type : CommentIn

Informations de copyright

Copyright © 2021 The Author(s). Published by Elsevier Inc. All rights reserved.

Déclaration de conflit d'intérêts

Declaration of interests S.H. is a paid consultant to Pipeline Therapeutics.

Auteurs

Amanda Janesick (A)

Department of Otolaryngology - Head & Neck Surgery, Institute for Stem Cell Biology & Regenerative Medicine, Stanford University School of Medicine, Palo Alto, CA 94305, USA. Electronic address: janesick@stanford.edu.

Mirko Scheibinger (M)

Department of Otolaryngology - Head & Neck Surgery, Institute for Stem Cell Biology & Regenerative Medicine, Stanford University School of Medicine, Palo Alto, CA 94305, USA.

Nesrine Benkafadar (N)

Department of Otolaryngology - Head & Neck Surgery, Institute for Stem Cell Biology & Regenerative Medicine, Stanford University School of Medicine, Palo Alto, CA 94305, USA.

Sakin Kirti (S)

Department of Otolaryngology - Head & Neck Surgery, Institute for Stem Cell Biology & Regenerative Medicine, Stanford University School of Medicine, Palo Alto, CA 94305, USA; Case Western Reserve University, Cleveland, OH 44106, USA.

Daniel C Ellwanger (DC)

Department of Otolaryngology - Head & Neck Surgery, Institute for Stem Cell Biology & Regenerative Medicine, Stanford University School of Medicine, Palo Alto, CA 94305, USA; Genome Analysis Unit, Amgen Research, Amgen, Inc., South San Francisco, CA 94080, USA.

Stefan Heller (S)

Department of Otolaryngology - Head & Neck Surgery, Institute for Stem Cell Biology & Regenerative Medicine, Stanford University School of Medicine, Palo Alto, CA 94305, USA. Electronic address: hellers@stanford.edu.

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Classifications MeSH

questionsmedicales.fr › Eucaryotes › Animaux › Cell-type identity of the avian cochlea.
questionsmedicales.fr › Facteurs biologiques › Marqueurs biologiques › Cell-type identity of the avian cochlea.
questionsmedicales.fr › Eucaryotes › Animaux › Chordés › Vertébrés › Oiseaux › Poulets › Cell-type identity of the avian cochlea.
questionsmedicales.fr › Organes des sens › Oreille › Oreille interne › Cochlée › Cell-type identity of the avian cochlea.
questionsmedicales.fr › Tissus › Épithélium › Cell-type identity of the avian cochlea.
questionsmedicales.fr › Techniques d'investigation › Techniques génétiques › Analyse de profil d'expression de gènes › Cell-type identity of the avian cochlea.
questionsmedicales.fr › Phénomènes génétiques › Régulation de l'expression des gènes › Cell-type identity of the avian cochlea.
questionsmedicales.fr › Cellules › Neurones › Neurones afférents › Cellules réceptrices sensorielles › Mécanorécepteurs › Cellules neuroépithéliales › Cellules ciliées auditives › Cellules ciliées auditives internes › Cell-type identity of the avian cochlea.
questionsmedicales.fr › Cellules › Cellules épithéliales › Cellules de soutien du labyrinthe › Cell-type identity of the avian cochlea.
questionsmedicales.fr › Techniques d'investigation › Techniques génétiques › Analyse de séquence › Analyse de séquence d'ARN › RNA-Seq › Cell-type identity of the avian cochlea.
questionsmedicales.fr › Environnement et santé publique › Santé publique › Méthodes épidémiologiques › Méthodologie en recherche épidémiologique › Reproductibilité des résultats › Cell-type identity of the avian cochlea.
questionsmedicales.fr › Techniques d'investigation › Techniques cytologiques › Analyse sur cellule unique › Cell-type identity of the avian cochlea.