The Impact of Lower Limb Immobilization and Rehabilitation on Angiogenic Proteins and Capillarization in Skeletal Muscle.
Angiogenic Proteins
/ metabolism
Capillaries
/ physiology
Exercise
/ physiology
Healthy Volunteers
Humans
Immobilization
/ methods
Lower Extremity
/ physiology
Male
Muscle, Skeletal
/ physiology
Neovascularization, Physiologic
/ physiology
Oxygen Consumption
/ physiology
Thrombospondin 1
/ metabolism
Vascular Endothelial Growth Factor A
/ metabolism
Young Adult
Journal
Medicine and science in sports and exercise
ISSN: 1530-0315
Titre abrégé: Med Sci Sports Exerc
Pays: United States
ID NLM: 8005433
Informations de publication
Date de publication:
01 09 2021
01 09 2021
Historique:
pubmed:
1
4
2021
medline:
25
11
2021
entrez:
31
3
2021
Statut:
ppublish
Résumé
Skeletal muscle vascularization is important for tissue regeneration after injury and immobilization. We examined whether complete immobilization influences capillarization and oxygen delivery to the muscle and assessed the efficacy of rehabilitation by aerobic exercise training. Young healthy males had one leg immobilized for 14 d and subsequently completed 4 wk of intense aerobic exercise training. Biopsies were obtained from musculus vastus lateralis, and arteriovenous blood sampling for assessment of oxygen extraction and leg blood flow during exercise was done before and after immobilization and training. Muscle capillarization, muscle and platelet content of vascular endothelial growth factor (VEGF), and muscle thrombospondin-1 were determined. Immobilization did not have a significant impact on capillary per fiber ratio or capillary density. The content of VEGF protein in muscle samples was reduced by 36% (P = 0.024), and VEGF to thrombospondin-1 ratio was 94% lower (P = 0.046). The subsequent 4-wk training period increased the muscle VEGF content and normalized the muscle VEGF to thrombospondin-1 ratio but did not influence capillarization. Platelet VEGF content followed the trend of muscle VEGF. At the functional level, oxygen extraction, blood flow, and oxygen delivery at rest and during submaximal exercise were not affected by immobilization or training. The results demonstrate that just 2 wk of leg immobilization leads to a strongly reduced angiogenic potential as evidenced by reduced muscle and platelet VEGF content and a reduced muscle VEGF to thrombospondin-1 ratio. Moreover, a subsequent period of intensive aerobic exercise training fails to increase capillarization in the previously immobilized leg, possibly because of the angiostatic condition caused by immobilization.
Identifiants
pubmed: 33787530
doi: 10.1249/MSS.0000000000002665
pii: 00005768-202109000-00001
doi:
Substances chimiques
Angiogenic Proteins
0
Thrombospondin 1
0
Vascular Endothelial Growth Factor A
0
Banques de données
ClinicalTrials.gov
['NCT03054376']
Types de publication
Journal Article
Research Support, Non-U.S. Gov't
Langues
eng
Sous-ensembles de citation
IM
Pagination
1797-1806Informations de copyright
Copyright © 2021 by the American College of Sports Medicine.
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