Production of nonulosonic acids in the extracellular polymeric substances of "Candidatus Accumulibacter phosphatis".
Biological phosphate removal
Extracellular polymeric substances
Granular sludge
Nonulosonic acids
Sialic acids
“Candidatus Accumulibacter phosphatis”
Journal
Applied microbiology and biotechnology
ISSN: 1432-0614
Titre abrégé: Appl Microbiol Biotechnol
Pays: Germany
ID NLM: 8406612
Informations de publication
Date de publication:
Apr 2021
Apr 2021
Historique:
received:
03
11
2020
accepted:
17
03
2021
revised:
02
02
2021
pubmed:
2
4
2021
medline:
15
5
2021
entrez:
1
4
2021
Statut:
ppublish
Résumé
Nonulosonic acids (NulOs) are a family of acidic carbohydrates with a nine-carbon backbone, which include different related structures, such as sialic acids. They have mainly been studied for their relevance in animal cells and pathogenic bacteria. Recently, sialic acids have been discovered as an important compound in the extracellular matrix of virtually all microbial life and in "Candidatus Accumulibacter phosphatis", a well-studied polyphosphate-accumulating organism, in particular. Here, bioaggregates highly enriched with these bacteria (approx. 95% based on proteomic data) were used to study the production of NulOs in an enrichment of this microorganism. Fluorescence lectin-binding analysis, enzymatic quantification, and mass spectrometry were used to analyze the different NulOs present, showing a wide distribution and variety of these carbohydrates, such as sialic acids and bacterial NulOs, in the bioaggregates. Phylogenetic analysis confirmed the potential of "Ca. Accumulibacter" to produce different types of NulOs. Proteomic analysis showed the ability of "Ca. Accumulibacter" to reutilize and reincorporate these carbohydrates. This investigation points out the importance of diverse NulOs in non-pathogenic bacteria, which are normally overlooked. Sialic acids and other NulOs should be further investigated for their role in the ecology of "Ca. Accumulibacter" in particular, and biofilms in general. KEY POINTS: •"Ca. Accumulibacter" has the potential to produce a range of nonulosonic acids. •Mass spectrometry and lectin binding can reveal the presence and location of nonulosonic acids. •The role of nonulosonic acid in non-pathogenic bacteria needs to be studied in detail.
Identifiants
pubmed: 33791836
doi: 10.1007/s00253-021-11249-3
pii: 10.1007/s00253-021-11249-3
pmc: PMC8053191
doi:
Substances chimiques
Sewage
0
Phosphorus
27YLU75U4W
Types de publication
Journal Article
Langues
eng
Sous-ensembles de citation
IM
Pagination
3327-3338Subventions
Organisme : Nederlandse Organisatie voor Wetenschappelijk Onderzoek
ID : ALWGK.2016.025
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