Chiral Transplacental Pharmacokinetics of Fexofenadine: Impact of P-Glycoprotein Inhibitor Fluoxetine Using the Human Placental Perfusion Model.


Journal

Pharmaceutical research
ISSN: 1573-904X
Titre abrégé: Pharm Res
Pays: United States
ID NLM: 8406521

Informations de publication

Date de publication:
Apr 2021
Historique:
received: 14 01 2021
accepted: 23 03 2021
pubmed: 8 4 2021
medline: 9 11 2021
entrez: 7 4 2021
Statut: ppublish

Résumé

Fexofenadine is a well-identified in vivo probe substrate of P-glycoprotein (P-gp) and/or organic anion transporting polypeptide (OATP). This work aimed to investigate the transplacental pharmacokinetics of fexofenadine enantiomers with and without the selective P-gp inhibitor fluoxetine. The chiral transplacental pharmacokinetics of fexofenadine-fluoxetine interaction was determined using the ex vivo human placenta perfusion model (n = 4). In the Control period, racemic fexofenadine (75 ng of each enantiomer/ml) was added in the maternal circuit. In the Interaction period, racemic fluoxetine (50 ng of each enantiomer/mL) and racemic fexofenadine (75 ng of each enantiomer/mL) were added to the maternal circulation. In both periods, maternal and fetal perfusate samples were taken over 90 min. The (S)-(-)- and (R)-(+)-fexofenadine fetal-to-maternal ratio values in Control and Interaction periods were similar (~0.18). The placental transfer rates were similar between (S)-(-)- and (R)-(+)-fexofenadine in both Control (0.0024 vs 0.0019 min Our study showed a low extent, slow rate of non-enantioselective placental transfer of fexofenadine enantiomers, indicating a limited fetal fexofenadine exposure mediated by placental P-gp and/or OATP2B1. The fluoxetine interaction did not affect the non-enantioselective transplacental transfer of fexofenadine. The ex vivo placental perfusion model accurately predicts in vivo placental transfer of fexofenadine enantiomers with remarkably similar values (~0.17), and thus estimates the limited fetal exposure.

Identifiants

pubmed: 33825113
doi: 10.1007/s11095-021-03035-7
pii: 10.1007/s11095-021-03035-7
doi:

Substances chimiques

ABCB1 protein, human 0
ATP Binding Cassette Transporter, Subfamily B 0
Histamine H1 Antagonists, Non-Sedating 0
Fluoxetine 01K63SUP8D
Terfenadine 7BA5G9Y06Q
fexofenadine E6582LOH6V

Types de publication

Journal Article

Langues

eng

Sous-ensembles de citation

IM

Pagination

647-655

Subventions

Organisme : Coordenação de Aperfeiçoamento de Pessoal de Nível Superior
ID : BEX6119/13-1

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Auteurs

Leonardo Pinto (L)

Department of Clinical Analysis, Food Science and Toxicology School of Pharmaceutical Sciences of Ribeirão Preto, University of São Paulo, Ribeirão Preto, SP, Brazil. leonardopinto84@gmail.com.
Division of Clinical Pharmacology and Toxicology, The Hospital for Sick Children, Toronto, Ontario, Canada. leonardopinto84@gmail.com.

Priya Bapat (P)

Division of Clinical Pharmacology and Toxicology, The Hospital for Sick Children, Toronto, Ontario, Canada.
Department of Pharmacology and Toxicology, University of Toronto, Toronto, Ontario, Canada.

Fernanda de Lima Moreira (F)

Department of Clinical Analysis, Food Science and Toxicology School of Pharmaceutical Sciences of Ribeirão Preto, University of São Paulo, Ribeirão Preto, SP, Brazil.

Angelika Lubetsky (A)

Division of Clinical Pharmacology and Toxicology, The Hospital for Sick Children, Toronto, Ontario, Canada.

Ricardo de Carvalho Cavalli (R)

Department of Obstetrics and Gynecology School of Medicine of Ribeirão Preto, University of São Paulo, Ribeirão Preto, SP, Brazil.

Howard Berger (H)

Department of Obstetrics and Gynecology, St. Michael's Hospital, Toronto, Ontario, Canada.

Vera Lucia Lanchote (VL)

Department of Clinical Analysis, Food Science and Toxicology School of Pharmaceutical Sciences of Ribeirão Preto, University of São Paulo, Ribeirão Preto, SP, Brazil.

Gideon Koren (G)

Adelson Faculty of Medicine, Ariel University, Ariel, Israel.
Motherisk Israel Program, Zerifn, Israel.

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