Application of a strain-level shotgun metagenomics approach on food samples: resolution of the source of a


Journal

Microbial genomics
ISSN: 2057-5858
Titre abrégé: Microb Genom
Pays: England
ID NLM: 101671820

Informations de publication

Date de publication:
04 2021
Historique:
entrez: 7 4 2021
pubmed: 8 4 2021
medline: 20 11 2021
Statut: ppublish

Résumé

Food-borne outbreak investigation currently relies on the time-consuming and challenging bacterial isolation from food, to be able to link food-derived strains to more easily obtained isolates from infected people. When no food isolate can be obtained, the source of the outbreak cannot be unambiguously determined. Shotgun metagenomics approaches applied to the food samples could circumvent this need for isolation from the suspected source, but require downstream strain-level data analysis to be able to accurately link to the human isolate. Until now, this approach has not yet been applied outside research settings to analyse real food-borne outbreak samples. In September 2019, a

Identifiants

pubmed: 33826490
doi: 10.1099/mgen.0.000547
pmc: PMC8208685
doi:

Types de publication

Evaluation Study Journal Article Research Support, Non-U.S. Gov't

Langues

eng

Sous-ensembles de citation

IM

Références

EFSA J. 2019 Dec 03;17(12):e05898
pubmed: 32626197
Microorganisms. 2020 Aug 05;8(8):
pubmed: 32764329
BMC Genomics. 2020 Feb 27;21(1):184
pubmed: 32106809
Microorganisms. 2020 Nov 25;8(12):
pubmed: 33255715
J Antimicrob Chemother. 2012 Nov;67(11):2640-4
pubmed: 22782487
J Med Microbiol. 2005 Jan;54(Pt 1):51-54
pubmed: 15591255
Food Res Int. 2019 Feb;116:802-809
pubmed: 30717011
JAMA. 2013 Apr 10;309(14):1502-10
pubmed: 23571589
Nat Methods. 2015 Aug;12(8):733-5
pubmed: 26076426
Appl Environ Microbiol. 2015 Dec;81(23):8183-91
pubmed: 26386062
Appl Microbiol Biotechnol. 2013 Nov;97(22):9811-24
pubmed: 24113820
Antimicrob Agents Chemother. 2013 Jul;57(7):3348-57
pubmed: 23650175
Mol Biol Evol. 2018 Jun 1;35(6):1547-1549
pubmed: 29722887
Nucleic Acids Res. 2016 Jul 8;44(W1):W242-5
pubmed: 27095192
Lancet Infect Dis. 2019 Jul;19(7):778-786
pubmed: 31133519
Genome Biol. 2020 Apr 28;21(1):103
pubmed: 32345331
Appl Environ Microbiol. 2017 Jan 17;83(3):
pubmed: 27881416
Clin Microbiol Infect. 2017 Jan;23(1):2-22
pubmed: 27890457
Appl Environ Microbiol. 2017 Aug 1;83(16):
pubmed: 28625983
Int J Food Microbiol. 2019 Jan 2;288:3-12
pubmed: 29803313
Genome Biol. 2015 May 30;16:114
pubmed: 26025440
Appl Environ Microbiol. 2018 Jan 31;84(4):
pubmed: 29196295
PLoS One. 2016 Jan 22;11(1):e0147101
pubmed: 26800248
EFSA J. 2019 Dec 11;17(12):e05926
pubmed: 32626211
PLoS One. 2016 Dec 8;11(12):e0167870
pubmed: 27930729
Mol Biol Evol. 2020 May 1;37(5):1530-1534
pubmed: 32011700
J Comput Biol. 2012 May;19(5):455-77
pubmed: 22506599
Front Microbiol. 2019 Mar 06;10:362
pubmed: 30894839
Front Microbiol. 2019 Jul 12;10:1591
pubmed: 31354679
Bioinformatics. 2013 Apr 15;29(8):1072-5
pubmed: 23422339
Bioinformatics. 2015 Jan 15;31(2):170-7
pubmed: 25266224
PLoS One. 2013 Oct 28;8(10):e77302
pubmed: 24204795
Mol Biol Evol. 1993 May;10(3):512-26
pubmed: 8336541
Int J Mol Sci. 2020 Aug 08;21(16):
pubmed: 32784459
Rev Sci Tech. 2016 Apr;35(1):83-93
pubmed: 27217170
Nat Rev Genet. 2018 Jan;19(1):9-20
pubmed: 29129921
Appl Environ Microbiol. 2016 Apr 04;82(8):2433-2443
pubmed: 26873315
Foodborne Pathog Dis. 2019 Jul;16(7):474-479
pubmed: 31170005
Genome Biol. 2019 Nov 28;20(1):257
pubmed: 31779668

Auteurs

Florence E Buytaers (FE)

Department of Plant Biotechnology and Bioinformatics, Ghent University, Ghent, Belgium.
Transversal Activities in Applied Genomics, Sciensano, Brussels, Belgium.

Assia Saltykova (A)

Department of Plant Biotechnology and Bioinformatics, Ghent University, Ghent, Belgium.
Transversal Activities in Applied Genomics, Sciensano, Brussels, Belgium.

Wesley Mattheus (W)

National Reference Center for Salmonella and Shigella spp., Sciensano, Brussels, Belgium.

Bavo Verhaegen (B)

National Reference Laboratory for Salmonella and Food-Borne Infections, Food-Borne Pathogens, Sciensano, Brussels, Belgium.

Nancy H C Roosens (NHC)

Transversal Activities in Applied Genomics, Sciensano, Brussels, Belgium.

Kevin Vanneste (K)

Transversal Activities in Applied Genomics, Sciensano, Brussels, Belgium.

Valeska Laisnez (V)

Agentschap Zorg en Gezondheid, Brussels, Belgium.

Naïma Hammami (N)

Agentschap Zorg en Gezondheid, Brussels, Belgium.

Brigitte Pochet (B)

Federal Agency for the Security of the Food Chain, Brussels, Belgium.

Vera Cantaert (V)

Federal Agency for the Security of the Food Chain, Brussels, Belgium.

Kathleen Marchal (K)

Department of Genetics, University of Pretoria, Pretoria, South Africa.
Department of Plant Biotechnology and Bioinformatics, Ghent University, Ghent, Belgium.
Department of Information Technology, IDlab, IMEC, Ghent University, Ghent, Belgium.

Sarah Denayer (S)

National Reference Laboratory for Salmonella and Food-Borne Infections, Food-Borne Pathogens, Sciensano, Brussels, Belgium.

Sigrid C J De Keersmaecker (SCJ)

Transversal Activities in Applied Genomics, Sciensano, Brussels, Belgium.

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Classifications MeSH