Dynamic Control of Gene Expression with Riboregulated Switchable Feedback Promoters.
Escherichia coli
/ genetics
Feedback, Physiological
Gene Expression
Gene Expression Regulation, Bacterial
Genes, Bacterial
Metabolic Engineering
/ methods
Metabolic Networks and Pathways
/ genetics
Operon
Promoter Regions, Genetic
/ genetics
Quorum Sensing
/ genetics
Riboswitch
/ genetics
Synthetic Biology
/ methods
RNA
feedback
gene regulation
isoprenoids
metabolic engineering
quorum-sensing
Journal
ACS synthetic biology
ISSN: 2161-5063
Titre abrégé: ACS Synth Biol
Pays: United States
ID NLM: 101575075
Informations de publication
Date de publication:
21 05 2021
21 05 2021
Historique:
pubmed:
10
4
2021
medline:
21
12
2021
entrez:
9
4
2021
Statut:
ppublish
Résumé
One major challenge in synthetic biology is the deleterious impacts of cellular stress caused by expression of heterologous pathways, sensors, and circuits. Feedback control and dynamic regulation are broadly proposed strategies to mitigate this cellular stress by optimizing gene expression levels temporally and in response to biological cues. While a variety of approaches for feedback implementation exist, they are often complex and cannot be easily manipulated. Here, we report a strategy that uses RNA transcriptional regulators to integrate additional layers of control over the output of natural and engineered feedback responsive circuits. Called riboregulated switchable feedback promoters (rSFPs), these gene expression cassettes can be modularly activated using multiple mechanisms, from manual induction to autonomous quorum sensing, allowing control over the timing, magnitude, and autonomy of expression. We develop rSFPs in
Identifiants
pubmed: 33834762
doi: 10.1021/acssynbio.1c00015
pmc: PMC8141045
mid: NIHMS1696552
doi:
Substances chimiques
Riboswitch
0
Types de publication
Journal Article
Research Support, N.I.H., Extramural
Research Support, Non-U.S. Gov't
Research Support, U.S. Gov't, Non-P.H.S.
Langues
eng
Sous-ensembles de citation
IM
Pagination
1199-1213Subventions
Organisme : NIGMS NIH HHS
ID : T32 GM008449
Pays : United States
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