Developments in solution-state NMR yield broader and deeper views of the dynamic ensembles of nucleic acids.
Dynamic ensembles
Excited conformational states
Nucleic acids
Solution state NMR
Journal
Current opinion in structural biology
ISSN: 1879-033X
Titre abrégé: Curr Opin Struct Biol
Pays: England
ID NLM: 9107784
Informations de publication
Date de publication:
10 2021
10 2021
Historique:
received:
15
02
2021
accepted:
20
02
2021
pubmed:
10
4
2021
medline:
4
11
2021
entrez:
9
4
2021
Statut:
ppublish
Résumé
Nucleic acids do not fold into a single conformation, and dynamic ensembles are needed to describe their propensities to cycle between different conformations when performing cellular functions. We review recent advances in solution-state nuclear magnetic resonance (NMR) methods and their integration with computational techniques that are improving the ability to probe the dynamic ensembles of DNA and RNA. These include computational approaches for predicting chemical shifts from structure and generating conformational libraries from sequence, measurements of exact nuclear Overhauser effects, development of new probes to study chemical exchange using relaxation dispersion, faster and more sensitive real-time NMR techniques, and new NMR approaches to tackle large nucleic acid assemblies. We discuss how these advances are leading to new mechanistic insights into gene expression and regulation.
Identifiants
pubmed: 33836446
pii: S0959-440X(21)00030-0
doi: 10.1016/j.sbi.2021.02.007
pmc: PMC8714323
mid: NIHMS1754619
pii:
doi:
Substances chimiques
Nucleic Acids
0
RNA
63231-63-0
DNA
9007-49-2
Types de publication
Journal Article
Research Support, N.I.H., Extramural
Review
Langues
eng
Sous-ensembles de citation
IM
Pagination
16-25Subventions
Organisme : NIAID NIH HHS
ID : U54 AI150470
Pays : United States
Organisme : NIGMS NIH HHS
ID : P50 GM103297
Pays : United States
Organisme : NIGMS NIH HHS
ID : R01 GM132899
Pays : United States
Organisme : NIAID NIH HHS
ID : R21 AI143460
Pays : United States
Organisme : NIAID NIH HHS
ID : R21 AI149112
Pays : United States
Organisme : NIGMS NIH HHS
ID : R01 GM089846
Pays : United States
Informations de copyright
Copyright © 2021 Elsevier Ltd. All rights reserved.
Déclaration de conflit d'intérêts
Conflict of interest statement HMA is an adviser to and holds an ownership interest in Nymirum Inc., an RNA-based drug discovery company. The other authors declare no competing interests.
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