RNA Sequencing and Pathways Analyses of Middle Ear Epithelia From Patients With Otitis Media.
Audiometry
Biopsy
Case-Control Studies
Child
Child, Preschool
Ear, Middle
/ pathology
Epithelium
/ pathology
Female
Gene Regulatory Networks
/ immunology
Genetic Predisposition to Disease
Healthy Volunteers
Humans
Infant
Male
Middle Ear Ventilation
Otitis Media
/ diagnosis
Protein Interaction Maps
/ genetics
RNA-Seq
Severity of Illness Index
Otitis media
RNA sequencing
RNA-Seq
otitis media with effusion
Journal
The Laryngoscope
ISSN: 1531-4995
Titre abrégé: Laryngoscope
Pays: United States
ID NLM: 8607378
Informations de publication
Date de publication:
11 2021
11 2021
Historique:
revised:
04
03
2021
received:
19
01
2021
accepted:
23
03
2021
pubmed:
13
4
2021
medline:
25
11
2021
entrez:
12
4
2021
Statut:
ppublish
Résumé
Otitis media (OM) is the most common pediatric diagnosis in the United States. However, our understanding of the molecular pathogenesis of OM remains relatively poor. Investigation of molecular pathways involved in OM may improve the understanding of this disease process and elucidate novel therapeutic targets. In this study, RNA sequencing (RNA-Seq) was used to discern cellular changes associated with OME compared to healthy middle ear epithelium (MEE). Ex vivo case-control translational. Middle ear epithelia was collected from five pediatric patients diagnosed with OME undergoing tympanostomy tube placement and five otherwise healthy pediatric patients undergoing cochlear implantation. Specimens underwent RNA-Seq and pathways analyses. A total of 1,292 genes exhibited differential expression in MEE from OME patients compared to controls including genes involved in inflammation, immune response to bacterial OM pathogens, mucociliary clearance, regulation of proliferation and transformation, and auditory cell differentiation. Top networks identified in OME were organismal injury and abnormalities, cell morphology, and auditory disease. Top Ingenuity canonical pathways identified were axonal guidance signaling, which contains genes associated with auditory development and disease and nicotine degradation II and III pathways. Associated upstream regulators included β-estradiol, dexamethasone, and G-protein-coupled estrogen receptor-1 (GPER1), which are associated with otoprotection or inflammation during insult. RNA-Seq demonstrates differential gene expression in MEE from patients with OME compared to healthy controls with important implications for infection susceptibility, hearing loss, and a role for tobacco exposure in the development and/or severity of OME in pediatric patients. 4 Laryngoscope, 131:2590-2597, 2021.
Types de publication
Journal Article
Observational Study
Research Support, N.I.H., Extramural
Research Support, Non-U.S. Gov't
Langues
eng
Sous-ensembles de citation
IM
Pagination
2590-2597Subventions
Organisme : Medical College of Wisconsin Genomic Sciences and Precision Medicine Center
Organisme : NIDCD NIH HHS
ID : 5R21DC015634-02
Pays : United States
Organisme : Genomic Sciences and Precision Medicine Center at the Medical College of Wisconsin
Organisme : National Institutes of Health Institute on Deafness and Communication Disorders
ID : 5R21DC015634-02
Organisme : Dr. Raul Urrutia of the Genomic Sciences and Precision Medicine Center at the Medical College of Wisconsin
Organisme : NIDCD NIH HHS
ID : 5R21DC015634-02
Pays : United States
Informations de copyright
© 2021 The American Laryngological, Rhinological and Otological Society, Inc.
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