A translational riboswitch coordinates nascent transcription-translation coupling.


Journal

Proceedings of the National Academy of Sciences of the United States of America
ISSN: 1091-6490
Titre abrégé: Proc Natl Acad Sci U S A
Pays: United States
ID NLM: 7505876

Informations de publication

Date de publication:
20 04 2021
Historique:
entrez: 14 4 2021
pubmed: 15 4 2021
medline: 15 12 2021
Statut: ppublish

Résumé

Bacterial messenger RNA (mRNA) synthesis by RNA polymerase (RNAP) and first-round translation by the ribosome are often coupled to regulate gene expression, yet how coupling is established and maintained is ill understood. Here, we develop biochemical and single-molecule fluorescence approaches to probe the dynamics of RNAP-ribosome interactions on an mRNA with a translational preQ

Identifiants

pubmed: 33850018
pii: 2023426118
doi: 10.1073/pnas.2023426118
pmc: PMC8072259
pii:
doi:

Substances chimiques

5' Untranslated Regions 0
Escherichia coli Proteins 0
NusG protein, E coli 0
Peptide Elongation Factors 0
RNA, Bacterial 0
RNA, Messenger 0
RfaH protein, E coli 0
Riboswitch 0
Trans-Activators 0
Transcription Factors 0
DNA-Directed RNA Polymerases EC 2.7.7.6

Types de publication

Journal Article Research Support, N.I.H., Extramural

Langues

eng

Sous-ensembles de citation

IM

Subventions

Organisme : NIGMS NIH HHS
ID : R01 GM062357
Pays : United States
Organisme : NIGMS NIH HHS
ID : R01 GM067153
Pays : United States
Organisme : NIGMS NIH HHS
ID : R01 GM118524
Pays : United States
Organisme : NIGMS NIH HHS
ID : R35 GM131922
Pays : United States

Commentaires et corrections

Type : CommentIn

Déclaration de conflit d'intérêts

The authors declare no competing interest.

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Auteurs

Surajit Chatterjee (S)

Single Molecule Analysis Group, Department of Chemistry and Center for RNA Biomedicine, University of Michigan, Ann Arbor, MI 48109.

Adrien Chauvier (A)

Single Molecule Analysis Group, Department of Chemistry and Center for RNA Biomedicine, University of Michigan, Ann Arbor, MI 48109.

Shiba S Dandpat (SS)

Single Molecule Analysis Group, Department of Chemistry and Center for RNA Biomedicine, University of Michigan, Ann Arbor, MI 48109.

Irina Artsimovitch (I)

Department of Microbiology, The Ohio State University, Columbus, OH 43210.

Nils G Walter (NG)

Single Molecule Analysis Group, Department of Chemistry and Center for RNA Biomedicine, University of Michigan, Ann Arbor, MI 48109; nwalter@umich.edu.

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Classifications MeSH