Mediator subunit Med15 dictates the conserved "fuzzy" binding mechanism of yeast transcription activators Gal4 and Gcn4.
Basic-Leucine Zipper Transcription Factors
/ chemistry
DNA-Binding Proteins
/ chemistry
Mediator Complex
/ chemistry
Methyltransferases
/ chemistry
Models, Molecular
Protein Interaction Domains and Motifs
Repressor Proteins
/ chemistry
Saccharomyces cerevisiae
/ genetics
Saccharomyces cerevisiae Proteins
/ chemistry
Transcription Factors
/ genetics
Journal
Nature communications
ISSN: 2041-1723
Titre abrégé: Nat Commun
Pays: England
ID NLM: 101528555
Informations de publication
Date de publication:
13 04 2021
13 04 2021
Historique:
received:
14
11
2019
accepted:
11
03
2021
entrez:
14
4
2021
pubmed:
15
4
2021
medline:
29
4
2021
Statut:
epublish
Résumé
The acidic activation domain (AD) of yeast transcription factor Gal4 plays a dual role in transcription repression and activation through binding to Gal80 repressor and Mediator subunit Med15. The activation function of Gal4 arises from two hydrophobic regions within the 40-residue AD. We show by NMR that each AD region binds the Mediator subunit Med15 using a "fuzzy" protein interface. Remarkably, comparison of chemical shift perturbations shows that Gal4 and Gcn4, two intrinsically disordered ADs of different sequence, interact nearly identically with Med15. The finding that two ADs of different sequence use an identical fuzzy binding mechanism shows a common sequence-independent mechanism for AD-Mediator binding, similar to interactions within a hydrophobic cloud. In contrast, the same region of Gal4 AD interacts strongly with Gal80 via a distinct structured complex, implying that the structured binding partner of an intrinsically disordered protein dictates the type of protein-protein interaction.
Identifiants
pubmed: 33850123
doi: 10.1038/s41467-021-22441-4
pii: 10.1038/s41467-021-22441-4
pmc: PMC8044209
doi:
Substances chimiques
Basic-Leucine Zipper Transcription Factors
0
DNA-Binding Proteins
0
GAL11 protein, S cerevisiae
0
GAL4 protein, S cerevisiae
0
GAL80 protein, S cerevisiae
0
GCN4 protein, S cerevisiae
0
Mediator Complex
0
Repressor Proteins
0
Saccharomyces cerevisiae Proteins
0
Transcription Factors
0
Methyltransferases
EC 2.1.1.-
mRNA (guanine(N7))-methyltransferase
EC 2.1.1.56
Types de publication
Journal Article
Research Support, N.I.H., Extramural
Langues
eng
Sous-ensembles de citation
IM
Pagination
2220Subventions
Organisme : NIGMS NIH HHS
ID : R01 GM075114
Pays : United States
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