Gene tagging via CRISPR-mediated homology-directed repair in cassava.
Xanthomonas
bacterial pathogens
cassava
genome editing
pathogenesis
Journal
G3 (Bethesda, Md.)
ISSN: 2160-1836
Titre abrégé: G3 (Bethesda)
Pays: England
ID NLM: 101566598
Informations de publication
Date de publication:
15 04 2021
15 04 2021
Historique:
received:
07
10
2020
accepted:
21
01
2021
pubmed:
16
4
2021
medline:
8
7
2021
entrez:
15
4
2021
Statut:
ppublish
Résumé
Research on a few model plant-pathogen systems has benefitted from years of tool and resource development. This is not the case for the vast majority of economically and nutritionally important plants, creating a crop improvement bottleneck. Cassava bacterial blight (CBB), caused by Xanthomonas axonopodis pv. manihotis (Xam), is an important disease in all regions where cassava (Manihot esculenta Crantz) is grown. Here, we describe the development of cassava that can be used to visualize one of the initial steps of CBB infection in vivo. Using CRISPR-mediated homology-directed repair (HDR), we generated plants containing scarless insertion of GFP at the 3' end of CBB susceptibility (S) gene MeSWEET10a. Activation of MeSWEET10a-GFP by the transcription activator-like (TAL) effector TAL20 was subsequently visualized at transcriptional and translational levels. To our knowledge, this is the first such demonstration of HDR via gene editing in cassava.
Identifiants
pubmed: 33855431
pii: 6126487
doi: 10.1093/g3journal/jkab028
pmc: PMC8049417
pii:
doi:
Banques de données
figshare
['10.25387/g3.13641035']
Types de publication
Journal Article
Research Support, Non-U.S. Gov't
Research Support, U.S. Gov't, Non-P.H.S.
Langues
eng
Sous-ensembles de citation
IM
Informations de copyright
© The Author(s) 2021. Published by Oxford University Press on behalf of Genetics Society of America.