Solvent-Dependent Stabilization of a Charge Transfer State is the Key to Ultrafast Triplet State Formation in an Epigenetic DNA Nucleoside.

DNA photophysics and photochemistry charge transfer states epigenetic nucleoside intersystem crossing triplet excited states

Journal

Chemistry (Weinheim an der Bergstrasse, Germany)
ISSN: 1521-3765
Titre abrégé: Chemistry
Pays: Germany
ID NLM: 9513783

Informations de publication

Date de publication:
26 Jul 2021
Historique:
received: 03 03 2021
pubmed: 17 4 2021
medline: 29 7 2021
entrez: 16 4 2021
Statut: ppublish

Résumé

2'-Deoxy-5-formylcytidine (5fdCyd), a naturally occurring nucleoside found in mammalian DNA and mitochondrial RNA, exhibits important epigenetic functionality in biological processes. Because it efficiently generates triplet excited states, it is an endogenous photosensitizer capable of damaging DNA, but the intersystem crossing (ISC) mechanism responsible for ultrafast triplet state generation is poorly understood. In this study, time-resolved mid-IR spectroscopy and quantum mechanical calculations reveal the distinct ultrafast ISC mechanisms of 5fdCyd in water versus acetonitrile. Our experiment indicates that in water, ISC to triplet states occurs within 1 ps after 285 nm excitation. PCM-TD-DFT computations suggest that this ultrafast ISC is mediated by a singlet state with significant cytosine-to-formyl charge-transfer (CT) character. In contrast, ISC in acetonitrile proceeds via a dark

Identifiants

pubmed: 33860588
doi: 10.1002/chem.202100787
doi:

Substances chimiques

Nucleosides 0
Solvents 0
DNA 9007-49-2

Types de publication

Journal Article

Langues

eng

Sous-ensembles de citation

IM

Pagination

10932-10940

Subventions

Organisme : National Natural Science Foundation of China
ID : 21873030
Organisme : National Natural Science Foundation of China
ID : 11674101
Organisme : National Natural Science Foundation of China
ID : 91850202
Organisme : Shanghai Rising-Star Program
ID : 19QA1402800
Organisme : National Science Foundation
ID : CHE-1800471
Organisme : MICINN project
ID : PID2019-110091GB-I00

Informations de copyright

© 2021 Wiley-VCH GmbH.

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Auteurs

Xueli Wang (X)

State Key Laboratory of Precision Spectroscopy, East China Normal University, Shanghai, 200241, P. R. China.

Lara Martínez-Fernández (L)

Departamento de Química, Facultad de Ciencias and Institute for Advanced Research in Chemistry (IADCHEM), Universidad Autónoma de Madrid Campus de Excelencia UAM-CSIC Cantoblanco, 28049, Madrid, Spain.

Yuyuan Zhang (Y)

Department of Chemistry and Biochemistry, The Ohio State University, 100 West 18th Avenue, Columbus, Ohio, 43210, USA.

Kun Zhang (K)

State Key Laboratory of Precision Spectroscopy, East China Normal University, Shanghai, 200241, P. R. China.

Roberto Improta (R)

Istituto di Biostrutture e Bioimmagini CNR, Via Mezzocannone 16, 80134, Napoli, Italy.

Bern Kohler (B)

Department of Chemistry and Biochemistry, The Ohio State University, 100 West 18th Avenue, Columbus, Ohio, 43210, USA.

Jianhua Xu (J)

State Key Laboratory of Precision Spectroscopy, East China Normal University, Shanghai, 200241, P. R. China.
Collaborative Innovation Center of Extreme Optics, Shanxi University, Taiyuan, Shanxi, 030006, P. R. China.

Jinquan Chen (J)

State Key Laboratory of Precision Spectroscopy, East China Normal University, Shanghai, 200241, P. R. China.
Collaborative Innovation Center of Extreme Optics, Shanxi University, Taiyuan, Shanxi, 030006, P. R. China.

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