Antibiotic susceptibility and genetic relatedness of Shigella species isolated from food and human stool samples in Qazvin, Iran.
Anti-Bacterial Agents
/ pharmacology
Child, Preschool
Cross-Sectional Studies
DNA, Bacterial
/ genetics
Diarrhea
/ drug therapy
Dysentery
/ drug therapy
Feces
/ microbiology
Food Contamination
/ analysis
Food Microbiology
/ methods
Foodborne Diseases
/ microbiology
Humans
Iran
/ epidemiology
Microbial Sensitivity Tests
/ methods
Polymerase Chain Reaction
Random Amplified Polymorphic DNA Technique
Shigella
/ drug effects
Antimicrobial susceptibility
Food samples
Genetic relatedness
Shigella species
Stool specimens
Journal
BMC research notes
ISSN: 1756-0500
Titre abrégé: BMC Res Notes
Pays: England
ID NLM: 101462768
Informations de publication
Date de publication:
17 Apr 2021
17 Apr 2021
Historique:
received:
02
02
2021
accepted:
02
04
2021
entrez:
18
4
2021
pubmed:
19
4
2021
medline:
22
4
2021
Statut:
epublish
Résumé
The aim of this study was to investigate the genetic relatedness and antimicrobial resistance among Shigella species isolated from food and stool samples. Using cross sectional study method, Shigella spp. were isolated from food and clinical samples using culture-based, biochemical and serological methods. Antimicrobial susceptibility and genetic relatedness among the isolates were evaluated using disk diffusion and RAPD-PCR methods respectively. The prevalence of Shigella spp. were 4.84 and 7.7% in food and stool samples respectively. All food isolates were Sh. sonnei. 91.42% of the Shigella stool isolates were Sh. sonnei. 62.5% of food isolates were resistant to tetracycline. 46.8, 50 and 65.8% of clinical isolates were resistant to imipenem, amikacin and azithromycin respectively. 50 and 85.7% of the food and clinical isolates respectively were MDR. Dendrogram generated by RAPD-PCR showed that the isolates from food and stool samples were categorized in a same group. Close genetic relatedness between MDR Shigella isolates from food and clinical samples indicate that foods can be considered as one of the main vehicles for transmission of MDR Shigella to human causing acute diseases. Survey of MDR Shigella among food and clinical samples is strongly suggested to be implemented.
Identifiants
pubmed: 33865447
doi: 10.1186/s13104-021-05554-3
pii: 10.1186/s13104-021-05554-3
pmc: PMC8052664
doi:
Substances chimiques
Anti-Bacterial Agents
0
DNA, Bacterial
0
Types de publication
Journal Article
Langues
eng
Sous-ensembles de citation
IM
Pagination
144Subventions
Organisme : Qazvin University of Medical Sciences
ID : 14003060
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