Single-cell sequencing of human white adipose tissue identifies new cell states in health and obesity.
Abdominoplasty
Adipocytes
/ immunology
Adult
Cell Communication
/ immunology
Cell Line
Dendritic Cells, Follicular
/ immunology
Female
Humans
Immunity, Innate
Inflammation
/ immunology
Lymphocytes
/ immunology
Macrophages
/ immunology
Obesity
/ immunology
RNA-Seq
Signal Transduction
/ immunology
Single-Cell Analysis
Subcutaneous Fat, Abdominal
/ immunology
Journal
Nature immunology
ISSN: 1529-2916
Titre abrégé: Nat Immunol
Pays: United States
ID NLM: 100941354
Informations de publication
Date de publication:
05 2021
05 2021
Historique:
received:
14
09
2020
accepted:
22
03
2021
pubmed:
29
4
2021
medline:
22
7
2021
entrez:
28
4
2021
Statut:
ppublish
Résumé
White adipose tissue (WAT) is an essential regulator of energy storage and systemic metabolic homeostasis. Regulatory networks consisting of immune and structural cells are necessary to maintain WAT metabolism, which can become impaired during obesity in mammals. Using single-cell transcriptomics and flow cytometry, we unveil a large-scale comprehensive cellular census of the stromal vascular fraction of healthy lean and obese human WAT. We report new subsets and developmental trajectories of adipose-resident innate lymphoid cells, dendritic cells and monocyte-derived macrophage populations that accumulate in obese WAT. Analysis of cell-cell ligand-receptor interactions and obesity-enriched signaling pathways revealed a switch from immunoregulatory mechanisms in lean WAT to inflammatory networks in obese WAT. These results provide a detailed and unbiased cellular landscape of homeostatic and inflammatory circuits in healthy human WAT.
Identifiants
pubmed: 33907320
doi: 10.1038/s41590-021-00922-4
pii: 10.1038/s41590-021-00922-4
pmc: PMC8102391
mid: NIHMS1686442
doi:
Types de publication
Journal Article
Research Support, N.I.H., Extramural
Research Support, Non-U.S. Gov't
Langues
eng
Sous-ensembles de citation
IM
Pagination
639-653Subventions
Organisme : NIAID NIH HHS
ID : T32 AI007323
Pays : United States
Organisme : NIDDK NIH HHS
ID : P30 DK063491
Pays : United States
Organisme : NIAID NIH HHS
ID : R01 AI145997
Pays : United States
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