Analysis of Intact Glycoproteins by Matrix-Assisted Laser Desorption/Ionization Time-of-Flight Mass Spectrometry.
Glycosylation degree
Intact glycoprotein
Ionic liquid
MALDI
Matrix
Journal
Methods in molecular biology (Clifton, N.J.)
ISSN: 1940-6029
Titre abrégé: Methods Mol Biol
Pays: United States
ID NLM: 9214969
Informations de publication
Date de publication:
2021
2021
Historique:
entrez:
28
4
2021
pubmed:
29
4
2021
medline:
24
6
2021
Statut:
ppublish
Résumé
Matrix-assisted laser desorption/ionization time-of-flight mass spectrometry (MALDI-TOF MS) can be regarded as a key tool to rapidly obtain molecular mass information of intact glycoproteins in glycoproteomic studies and quality control of recombinant biopharmaceuticals. However, MALDI-TOF MS of these glycosylated compounds is a tricky task due to its low ionization efficiency and fragmentation of labile groups such as sialic acids.Here, we offer the reader a practical overview of the available methodologies for the confident analysis of intact glycoproteins with different glycosylation degree by MALDI-TOF MS. The three proposed methods fulfil the requirements of reproducibility and low extent of glycan fragmentation required to successfully analyze intact glycoproteins.
Identifiants
pubmed: 33907998
doi: 10.1007/978-1-0716-1241-5_3
doi:
Substances chimiques
Glycoproteins
0
Types de publication
Journal Article
Research Support, Non-U.S. Gov't
Review
Langues
eng
Sous-ensembles de citation
IM
Pagination
47-56Références
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