Mechanochemical properties of DNA origami nanosprings revealed by force jumps in optical tweezers.
Journal
Nanoscale
ISSN: 2040-3372
Titre abrégé: Nanoscale
Pays: England
ID NLM: 101525249
Informations de publication
Date de publication:
14 May 2021
14 May 2021
Historique:
pubmed:
29
4
2021
medline:
20
5
2021
entrez:
28
4
2021
Statut:
ppublish
Résumé
By incorporating pH responsive i-motif elements, we have constructed DNA origami nanosprings that respond to pH changes in the environment. Using an innovative force jump approach in optical tweezers, we have directly measured the spring constants and dynamic recoiling responses of the DNA nanosprings under different forces. These DNA nanosprings exhibited 3 times slower recoiling rates compared to duplex DNA backbones. In addition, we observed two distinct force regions which show different spring constants. In the entropic region below 2 pN, a spring constant of ∼0.03 pN nm
Identifiants
pubmed: 33908965
doi: 10.1039/d0nr08605c
pmc: PMC8170849
mid: NIHMS1698711
doi:
Substances chimiques
DNA
9007-49-2
Types de publication
Journal Article
Langues
eng
Sous-ensembles de citation
IM
Pagination
8425-8430Subventions
Organisme : NCI NIH HHS
ID : R01 CA236350
Pays : United States
Références
Annu Rev Physiol. 2018 Feb 10;80:353-387
pubmed: 29433414
Chem Rev. 2017 Oct 25;117(20):12584-12640
pubmed: 28605177
J Am Chem Soc. 2010 Jul 7;132(26):8991-7
pubmed: 20545340
Biophys J. 1997 Mar;72(3):1335-46
pubmed: 9138579
Microsc Res Tech. 2011 Jul;74(7):688-98
pubmed: 21698717
Proc Natl Acad Sci U S A. 2018 Sep 18;115(38):9539-9544
pubmed: 30181280
Science. 2012 Feb 17;335(6070):831-4
pubmed: 22344439
Nat Chem. 2011 Aug 28;3(10):782-7
pubmed: 21941250
Cancer Res. 1989 Aug 15;49(16):4373-84
pubmed: 2545340
Science. 2011 Apr 15;332(6027):342-6
pubmed: 21493857
Nat Struct Biol. 1999 Apr;6(4):346-9
pubmed: 10201403
Biophys J. 2000 Apr;78(4):1965-78
pubmed: 10733975
Proc Natl Acad Sci U S A. 1997 May 13;94(10):4853-60
pubmed: 9144154
Science. 2009 Aug 7;325(5941):725-30
pubmed: 19661424
Annu Rev Biomed Eng. 2018 Jun 4;20:375-401
pubmed: 29618223
Science. 1996 Mar 29;271(5257):1835-7
pubmed: 8596951
Nat Commun. 2016 Dec 12;7:13715
pubmed: 27941751
Bioconjug Chem. 2021 Feb 17;32(2):311-317
pubmed: 33475341
Biophys J. 1999 Jan;76(1 Pt 1):409-13
pubmed: 9876152
Nature. 2009 May 21;459(7245):414-8
pubmed: 19458720
Nature. 1998 Aug 6;394(6693):539-44
pubmed: 9707114
Opt Lett. 1986 May 1;11(5):288
pubmed: 19730608