Nitric oxide donor sodium nitroprusside-induced transcriptional changes and hypocrellin biosynthesis of Shiraia sp. S9.
Hypocrellin
Nitric oxide
Oxidative stress
Shiraia
Sodium nitroprusside
Journal
Microbial cell factories
ISSN: 1475-2859
Titre abrégé: Microb Cell Fact
Pays: England
ID NLM: 101139812
Informations de publication
Date de publication:
28 Apr 2021
28 Apr 2021
Historique:
received:
15
12
2020
accepted:
17
04
2021
entrez:
29
4
2021
pubmed:
30
4
2021
medline:
15
10
2021
Statut:
epublish
Résumé
Nitric oxide (NO) is a ubiquitous signaling mediator in various physiological processes. However, there are less reports concerning the effects of NO on fungal secondary metabolites. Hypocrellins are effective anticancer photodynamic therapy (PDT) agents from fungal perylenequinone pigments of Shiraia. NO donor sodium nitroprusside (SNP) was used as a chemical elicitor to promote hypocrellin biosynthesis in Shiraia mycelium cultures. SNP application at 0.01-0.20 mM was found to stimulate significantly fungal production of perylenequinones including hypocrellin A (HA) and elsinochrome A (EA). SNP application could not only enhance HA content by 178.96% in mycelia, but also stimulate its efflux to the medium. After 4 days of SNP application at 0.02 mM, the highest total production (110.34 mg/L) of HA was achieved without any growth suppression. SNP released NO in mycelia and acted as a pro-oxidant, thereby up-regulating the gene expression and activity of reactive oxygen species (ROS) generating NADPH oxidase (NOX) and antioxidant enzymes, leading to the increased levels of superoxide anion (O The results of this study provide a valuable strategy for large-scale hypocrellin production and can facilitate further understanding and exploration of NO signaling in the biosynthesis of the important fungal metabolites.
Sections du résumé
BACKGROUND
BACKGROUND
Nitric oxide (NO) is a ubiquitous signaling mediator in various physiological processes. However, there are less reports concerning the effects of NO on fungal secondary metabolites. Hypocrellins are effective anticancer photodynamic therapy (PDT) agents from fungal perylenequinone pigments of Shiraia. NO donor sodium nitroprusside (SNP) was used as a chemical elicitor to promote hypocrellin biosynthesis in Shiraia mycelium cultures.
RESULTS
RESULTS
SNP application at 0.01-0.20 mM was found to stimulate significantly fungal production of perylenequinones including hypocrellin A (HA) and elsinochrome A (EA). SNP application could not only enhance HA content by 178.96% in mycelia, but also stimulate its efflux to the medium. After 4 days of SNP application at 0.02 mM, the highest total production (110.34 mg/L) of HA was achieved without any growth suppression. SNP released NO in mycelia and acted as a pro-oxidant, thereby up-regulating the gene expression and activity of reactive oxygen species (ROS) generating NADPH oxidase (NOX) and antioxidant enzymes, leading to the increased levels of superoxide anion (O
CONCLUSIONS
CONCLUSIONS
The results of this study provide a valuable strategy for large-scale hypocrellin production and can facilitate further understanding and exploration of NO signaling in the biosynthesis of the important fungal metabolites.
Identifiants
pubmed: 33910564
doi: 10.1186/s12934-021-01581-8
pii: 10.1186/s12934-021-01581-8
pmc: PMC8082767
doi:
Substances chimiques
Nitric Oxide Donors
0
Quinones
0
Reactive Oxygen Species
0
Nitroprusside
169D1260KM
Phenol
339NCG44TV
Perylene
5QD5427UN7
hypocrellin A
V86371B0XS
Types de publication
Journal Article
Langues
eng
Sous-ensembles de citation
IM
Pagination
92Subventions
Organisme : National Natural Science Foundation of China
ID : 82073955
Organisme : National Natural Science Foundation of China
ID : 81773695
Organisme : Priority Academic Program Development of the Jiangsu Higher Education Institutes
ID : PAPD
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