Bio-orthogonal chemistry enables solid phase synthesis and HPLC and gel-free purification of long RNA oligonucleotides.

Chromatography, High Pressure Liquid Nucleic Acid Conformation Oligonucleotides / chemical synthesis RNA / chemical synthesis Solid-Phase Synthesis Techniques

Journal

Chemical communications (Cambridge, England)
ISSN: 1364-548X
Titre abrégé: Chem Commun (Camb)
Pays: England
ID NLM: 9610838

Informations de publication

Date de publication:
29 Apr 2021
Historique:
entrez: 29 4 2021
pubmed: 30 4 2021
medline: 29 7 2021
Statut: ppublish

Résumé

Solid phase synthesis of RNA oligonucleotides which are over 100-nt in length remains challenging due to the complexity of purification of the target strand from failure sequences. This work describes a non-chromatographic strategy that will enable routine solid phase synthesis of long RNA strands.

Identifiants

DOI: 10.1039/d1cc00096a PMID: 33913974
pubmed: 33913974
doi: 10.1039/d1cc00096a
doi:

Substances chimiques

Oligonucleotides 0
RNA 63231-63-0

Types de publication

Journal Article

Langues

eng

Sous-ensembles de citation

IM

Pagination

4263-4266

Auteurs

Muhan He (M)

University at Albany, Department of Chemistry, Albany, NY,12222, USA. mroyzen@albany.edu.

Xunshen Wu (X)

University at Albany, Department of Chemistry, Albany, NY,12222, USA. mroyzen@albany.edu.

Song Mao (S)

University at Albany, Department of Chemistry, Albany, NY,12222, USA. mroyzen@albany.edu.

Phensinee Haruehanroengra (P)

University at Albany, Department of Chemistry, Albany, NY,12222, USA. mroyzen@albany.edu.

Irfan Khan (I)

University at Albany, Department of Chemistry, Albany, NY,12222, USA. mroyzen@albany.edu.
ORCID: 0000-0003-3854-5420

Jia Sheng (J)

University at Albany, Department of Chemistry, Albany, NY,12222, USA. mroyzen@albany.edu.
ORCID: 0000-0001-6198-390X

Maksim Royzen (M)

University at Albany, Department of Chemistry, Albany, NY,12222, USA. mroyzen@albany.edu.
ORCID: 0000-0002-2930-2171

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Classifications MeSH

questionsmedicales.fr › Techniques d'investigation › Techniques de chimie analytique › Chromatographie › Chromatographie en phase liquide › Chromatographie en phase liquide à haute performance › Bio-orthogonal chemistry enables solid phase...
questionsmedicales.fr › Phénomènes génétiques › Structures génétiques › Conformation d'acide nucléique › Bio-orthogonal chemistry enables solid phase...
questionsmedicales.fr › Acides nucléiques, nucléotides et nucléosides › Nucléotides › Polynucléotides › Oligonucléotides › Bio-orthogonal chemistry enables solid phase...
questionsmedicales.fr › Acides nucléiques, nucléotides et nucléosides › Acides nucléiques › ARN › Bio-orthogonal chemistry enables solid phase...
questionsmedicales.fr › Technologie, industrie et agriculture › Technologie › Technologie pharmaceutique › Techniques de chimie synthétique › Techniques de synthèse en phase solide › Bio-orthogonal chemistry enables solid phase...