A flow cytometric assay to detect platelet-activating antibodies in VITT after ChAdOx1 nCov-19 vaccination.
Antibody Specificity
Autoantibodies
/ biosynthesis
COVID-19
/ prevention & control
COVID-19 Vaccines
/ adverse effects
ChAdOx1 nCoV-19
Flow Cytometry
/ methods
Heparin
/ adverse effects
Humans
Immunoenzyme Techniques
Immunogenicity, Vaccine
Immunoglobulin G
/ biosynthesis
P-Selectin
/ analysis
Platelet Activation
/ immunology
Platelet Factor 4
/ immunology
Purpura, Thrombocytopenic, Idiopathic
/ diagnosis
Receptors, IgG
/ immunology
SARS-CoV-2
Vaccination
/ adverse effects
Journal
Blood
ISSN: 1528-0020
Titre abrégé: Blood
Pays: United States
ID NLM: 7603509
Informations de publication
Date de publication:
01 07 2021
01 07 2021
Historique:
received:
08
04
2021
accepted:
03
05
2021
pubmed:
5
5
2021
medline:
10
7
2021
entrez:
4
5
2021
Statut:
ppublish
Résumé
Vaccination is crucial in combatting the severe acute respiratory syndrome coronavirus 2 pandemic. The rare complication of thrombocytopenia and thrombotic complications at unusual sites after ChAdOx1 nCov-19 vaccination is caused by platelet-activating antibodies directed against platelet factor 4 (PF4). We present a widely applicable whole-blood standard flow cytometric assay to identify the pathogenic antibodies associated with vaccine-induced immune-mediated thrombotic thrombocytopenia (VITT) after ChAdOx1 nCov-19 vaccination. This assay will enable rapid diagnosis by many laboratories. This trial was registered at www.clinicaltrials.gov as #NCT04370119.
Identifiants
pubmed: 33945605
pii: S0006-4971(21)00986-1
doi: 10.1182/blood.2021012064
pmc: PMC8105122
doi:
Substances chimiques
Autoantibodies
0
COVID-19 Vaccines
0
Fc gamma receptor IIA
0
Immunoglobulin G
0
P-Selectin
0
PF4 protein, human
0
Receptors, IgG
0
SELP protein, human
0
Platelet Factor 4
37270-94-3
Heparin
9005-49-6
ChAdOx1 nCoV-19
B5S3K2V0G8
Banques de données
ClinicalTrials.gov
['NCT04370119']
Types de publication
Clinical Trial
Journal Article
Research Support, N.I.H., Extramural
Langues
eng
Sous-ensembles de citation
IM
Pagination
3656-3659Subventions
Organisme : NCATS NIH HHS
ID : UL1 TR001436
Pays : United States
Informations de copyright
© 2021 by The American Society of Hematology.
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