The Putative RNA-Binding Protein Dri1 Promotes the Loading of Kinesin-14/Klp2 to the Mitotic Spindle and Is Sequestered into Heat-Induced Protein Aggregates in Fission Yeast.
RNA-binding protein
fission yeast
heat stress
kinesin-14
mitotic spindle
Journal
International journal of molecular sciences
ISSN: 1422-0067
Titre abrégé: Int J Mol Sci
Pays: Switzerland
ID NLM: 101092791
Informations de publication
Date de publication:
30 Apr 2021
30 Apr 2021
Historique:
received:
15
03
2021
revised:
21
04
2021
accepted:
29
04
2021
entrez:
5
5
2021
pubmed:
6
5
2021
medline:
28
5
2021
Statut:
epublish
Résumé
Cells form a bipolar spindle during mitosis to ensure accurate chromosome segregation. Proper spindle architecture is established by a set of kinesin motors and microtubule-associated proteins. In most eukaryotes, kinesin-5 motors are essential for this process, and genetic or chemical inhibition of their activity leads to the emergence of monopolar spindles and cell death. However, these deficiencies can be rescued by simultaneous inactivation of kinesin-14 motors, as they counteract kinesin-5. We conducted detailed genetic analyses in fission yeast to understand the mechanisms driving spindle assembly in the absence of kinesin-5. Here, we show that deletion of the
Identifiants
pubmed: 33946513
pii: ijms22094795
doi: 10.3390/ijms22094795
pmc: PMC8125374
pii:
doi:
Substances chimiques
Cut7 protein, S pombe
0
Klp2 protein, S pombe
0
Microtubule-Associated Proteins
0
Protein Aggregates
0
Schizosaccharomyces pombe Proteins
0
Kinesins
EC 3.6.4.4
Types de publication
Journal Article
Langues
eng
Sous-ensembles de citation
IM
Subventions
Organisme : Japan Society for the Promotion of Science, KAKENHI Scientific Research (A)
ID : 16H02503
Organisme : Japan Society for the Promotion of Science, KAKENH Scientific Research (C)
ID : 19K05813
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