High-throughput measuring of meiotic recombination rates in barley pollen nuclei using Crystal Digital PCR
Hordeum vulgare
Crystal Digital PCRTM
barley
crossover
meiosis
meiotic recombination
single pollen nucleus genotyping
single-cell
technical advance
Journal
The Plant journal : for cell and molecular biology
ISSN: 1365-313X
Titre abrégé: Plant J
Pays: England
ID NLM: 9207397
Informations de publication
Date de publication:
07 2021
07 2021
Historique:
revised:
27
04
2021
received:
12
02
2021
accepted:
28
04
2021
pubmed:
6
5
2021
medline:
30
11
2021
entrez:
5
5
2021
Statut:
ppublish
Résumé
Breeding exploits novel allelic combinations assured by meiotic recombination. Barley (Hordeum vulgare) single pollen nucleus genotyping enables measurement of meiotic recombination rates in gametes before fertilization without the need for segregating populations. However, so far, established methods rely on whole-genome amplification of every single pollen nucleus due to their limited DNA content, thus restricting the number of analyzed samples. In this study, high-throughput measurements of meiotic recombination rates in barley pollen nuclei without whole-genome amplification were performed through a Crystal Digital PCR
Types de publication
Journal Article
Research Support, Non-U.S. Gov't
Langues
eng
Sous-ensembles de citation
IM
Pagination
649-661Informations de copyright
© 2021 The Authors. The Plant Journal published by Society for Experimental Biology and John Wiley & Sons Ltd.
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