Understanding 3D genome organization by multidisciplinary methods.

Chromatin / chemistry Chromosome Mapping Chromosomes / chemistry Computational Biology Genome High-Throughput Nucleotide Sequencing Humans Microscopy Models, Molecular Sequence Analysis, DNA

Journal

Nature reviews. Molecular cell biology
ISSN: 1471-0080
Titre abrégé: Nat Rev Mol Cell Biol
Pays: England
ID NLM: 100962782

Informations de publication

Date de publication:
08 2021
Historique:
accepted: 16 03 2021
pubmed: 7 5 2021
medline: 29 9 2021
entrez: 6 5 2021
Statut: ppublish

Résumé

Understanding how chromatin is folded in the nucleus is fundamental to understanding its function. Although 3D genome organization has been historically difficult to study owing to a lack of relevant methodologies, major technological breakthroughs in genome-wide mapping of chromatin contacts and advances in imaging technologies in the twenty-first century considerably improved our understanding of chromosome conformation and nuclear architecture. In this Review, we discuss methods of 3D genome organization analysis, including sequencing-based techniques, such as Hi-C and its derivatives, Micro-C, DamID and others; microscopy-based techniques, such as super-resolution imaging coupled with fluorescence in situ hybridization (FISH), multiplex FISH, in situ genome sequencing and live microscopy methods; and computational and modelling approaches. We describe the most commonly used techniques and their contribution to our current knowledge of nuclear architecture and, finally, we provide a perspective on up-and-coming methods that open possibilities for future major discoveries.

Identifiants

DOI: 10.1038/s41580-021-00362-w PMID: 33953379
pubmed: 33953379
doi: 10.1038/s41580-021-00362-w
pii: 10.1038/s41580-021-00362-w
doi:

Substances chimiques

Chromatin 0

Types de publication

Journal Article Research Support, Non-U.S. Gov't Review

Langues

eng

Sous-ensembles de citation

IM

Pagination

511-528

Informations de copyright

© 2021. Springer Nature Limited.

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Auteurs

Ivana Jerkovic (I)

Institute of Human Genetics, CNRS, University of Montpellier, Montpellier, France.
ORCID: 0000-0001-9707-8303

Giacomo Cavalli (G)

Institute of Human Genetics, CNRS, University of Montpellier, Montpellier, France. giacomo.cavalli@igh.cnrs.fr.
ORCID: 0000-0003-3709-3469

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questionsmedicales.fr › Phénomènes génétiques › Structures génétiques › Structures de chromosome › Chromatine › Understanding 3D genome organization by...
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