Overexpression of thioredoxin m in chloroplasts alters carbon and nitrogen partitioning in tobacco.
Carbon metabolism
GS-GOGAT pathway
chloroplast
glutamine synthetase
nitrogen metabolism
photorespiration
thioredoxin
Journal
Journal of experimental botany
ISSN: 1460-2431
Titre abrégé: J Exp Bot
Pays: England
ID NLM: 9882906
Informations de publication
Date de publication:
22 06 2021
22 06 2021
Historique:
received:
19
01
2021
accepted:
27
04
2021
pubmed:
9
5
2021
medline:
10
8
2021
entrez:
8
5
2021
Statut:
ppublish
Résumé
In plants, there is a complex interaction between carbon (C) and nitrogen (N) metabolism, and its coordination is fundamental for plant growth and development. Here, we studied the influence of thioredoxin (Trx) m on C and N partitioning using tobacco plants overexpressing Trx m from the chloroplast genome. The transgenic plants showed altered metabolism of C (lower leaf starch and soluble sugar accumulation) and N (with higher amounts of amino acids and soluble protein), which pointed to an activation of N metabolism at the expense of carbohydrates. To further delineate the effect of Trx m overexpression, metabolomic and enzymatic analyses were performed on these plants. These results showed an up-regulation of the glutamine synthetase-glutamate synthase pathway; specifically tobacco plants overexpressing Trx m displayed increased activity and stability of glutamine synthetase. Moreover, higher photorespiration and nitrate accumulation were observed in these plants relative to untransformed control plants, indicating that overexpression of Trx m favors the photorespiratory N cycle rather than primary nitrate assimilation. Taken together, our results reveal the importance of Trx m as a molecular mediator of N metabolism in plant chloroplasts.
Identifiants
pubmed: 33963398
pii: 6272276
doi: 10.1093/jxb/erab193
pmc: PMC8219043
doi:
Substances chimiques
Chloroplast Thioredoxins
0
Carbon
7440-44-0
Nitrogen
N762921K75
Types de publication
Journal Article
Research Support, Non-U.S. Gov't
Langues
eng
Sous-ensembles de citation
IM
Pagination
4949-4964Informations de copyright
© The Author(s) 2021. Published by Oxford University Press on behalf of the Society for Experimental Biology.
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