Effects of Amino Acids and Overexpression of dapA Gene on the Production of ε-Poly-L-lysine by Streptomyces diastatochromogenes Strains.
Journal
Current microbiology
ISSN: 1432-0991
Titre abrégé: Curr Microbiol
Pays: United States
ID NLM: 7808448
Informations de publication
Date de publication:
Jul 2021
Jul 2021
Historique:
received:
19
11
2020
accepted:
25
04
2021
pubmed:
16
5
2021
medline:
23
6
2021
entrez:
15
5
2021
Statut:
ppublish
Résumé
In this study, the strain Streptomyces diastatochromogenes 6#-7, which efficiently synthesizes ε-Poly-L-lysine, was studied and the effects of 18 amino acids and overexpression of dapA gene on the fermentation efficiency of ε-PL by S. diastatochromogenes were investigated. It was shown that L-proline, L-lysine, L-isoleucine, and L-threonine could promote the production of ε-PL. Moreover, the overexpression of the dihydrodipicolinate synthase gene (dapA) helped improve the fermentation performance of S. diastatochromogenes. The maximum ε-PL yield of the overexpressing strain (S. diastatochromogenes 12#-2) increased by 17.5% compared with the original strain in 500 mL shake flask. When the fermentation was conducted in a 5-L fermenter, the fermentation duration was extended by 48 h, and ε-PL yield reached 30.54 g/L, which was a 19.8% increase compared to the original strain. The results of this study offered a promising approach to augment the production of ε-PL from Streptomyces, thus paving the way to reduce the cost of product ε-PL and enhance the fermentation efficiency of ε-PL production.
Identifiants
pubmed: 33991200
doi: 10.1007/s00284-021-02510-z
pii: 10.1007/s00284-021-02510-z
doi:
Substances chimiques
Amino Acids
0
Polylysine
25104-18-1
Types de publication
Journal Article
Langues
eng
Sous-ensembles de citation
IM
Pagination
2640-2647Subventions
Organisme : National Key R and D Program of China
ID : NO. 2018YFD0400205
Organisme : National Natural Fund General Program of China
ID : NO. 31771952
Organisme : Open Project Program of State Key Laboratory of Food Nutrition and Safety
ID : No. SKLFNS-KF-201911
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